Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
101
Range %
98-105
1:2
Average %
85
Range %
81-89
1:4
Average %
87
Range %
82-90
1:8
Average %
93
Range %
90-96
回收率:
The recovery of bovine GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
96
93-99
EDTA plasma (n=4)
92
87-96
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Please provide the following information regarding Bovine growth hormone, GH ELISA Kit (CSB-E13443B) a. What is the standard? b. What is the antibody in this kit?
A:
Thanks for your inquiry. CSB-E13443B Standard:293 cell-derived recombinant protein; The plate is coated with goat anti-rabbit secondary antibody, and the specific antibody is rabbit polyclonal antibody. Pls let me know if you have any further questions. Thank you.
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能參考文獻:
Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells. PMID: 30011805
A microarray analysis showed that lncYYW positively regulates the expression of growth hormone 1 and its downstream genes, AKT1 and PIK3CD, in bovine myoblasts. This discovery provides a good foundation for further study of the mechanism of action of lncYYW during bovine myoblast development PMID: 28726188
bGH mice had increased body weight and decreased percent fat mass. Serum FGF21 levels were significantly elevated in bGH mice. Expression of Fgf21, Fgfr1, and Klb mRNA in white AT and liver were downregulated or unchanged in bGH mice. PMID: 27585733
GH/HpaII locus as candidate marker for body weight in cattle rather than MSTN/DraI. PMID: 29023020
An SNP GH4.1 in a growth hormone gene was significantly related to a weaning weight. PMID: 27706683
The association of IGF1, GH, and PIT1 markers with growth and reproductive traits were assessed. PMID: 25258122
Male bovine GH transgenic mice have decreased adiposity, and have increased regulatory T cells and macrophages in mesenteric and subcutaneous white adipose tissue. PMID: 25521584
The total frequencies for the combined genotypes for the bGH and RORC genes, which provide for superior meat quality and carcass weight, in the populations of Kazakh white-headed cattle PMID: 25975152
There is an association between a polymorphism in intron 3 of the BGH gene and both body weight at first estrus and first calving. PMID: 25158245
Whereas only moderate structural changes were observed with up-regulation of GH/IGF-I axis, disruption of the GH receptor had pronounced effects upon tendon ultra-structure. PMID: 24080228
this study identifies a biochemical mechanism for the regulation of SCFAs on bovine GH and PRL gene transcription in dairy cow anterior pituitary cells PMID: 24177567
homozygote genotypes of GH (LL) and beta-LG (AA) were superior compared to heterozygote genotypes, whereas, the heterozygote genotype of Pit-1 gene (AB) was desirable PMID: 22730770
one GH1 SNP, GH33, was significantly associated with milk protein yield in the second lactation. Several GH1 SNPs were significantly associated with fertility. PMID: 21948746
GH and IGF-I genotypes had no substantial effect on productive parameters, though IGF-I genotype affected calving-first service interval in primiparous cows. However, the genotypes do alter the endocrine/metabolic profiles of the transition dairy cow. PMID: 21635772
Association of cattle growth hormone gene polymorphism with milk productivity PMID: 21866862
Leptin modulation of lymphocytic growth hormone plays a role in the regulation of immune response during pregnancy. PMID: 21081139
Results further demonstrate the multifaceted influences of GH1 on milk production, fertility, and growth-related traits in cattle. PMID: 21094770
Study of genetic variation in Yakutian cattle (Bos taurus L.) using the prolactin bPRL, growth hormone bGH, and transcription factor bPit-1 genes PMID: 20391788
More than half of the CSF GHRH pulses observed in the current study were followed by episodes of GH release; leptin suppressed, while neuropeptide Y increased serum GH levels. PMID: 19632078
Findings suggest that the GH gene polymorphism at codons 127 and 172 was related to body weight, circulating basal GH and insulin, and GHRH-induced GH responses in Japanese Black heifers. PMID: 19747793
bGH-transgenic mice display marked changes in hepatic genes coding for metabolic enzymes and suggest that GH directly or indirectly regulates many of these hepatic genes via decreased expression of PPARalpha and SREBP-1. PMID: 12736163
GH administration had no effect on plasma leptin during late pregnancy or early lactation. PMID: 12881203
Hepatic growth hormone receptor(GHR) and IGF-I mRNA levels were strongly correlated with each other in chronic GH excess or deficiency. Regulation and correlation between GHR and IGF-I mRNA levels induced by GH is different between liver and pituitary. PMID: 14726438
Associations were analysed between polymorphisms of the growth hormone gene (GH-MspI) (localized in intron 3) and milk production traits of Beijing Holstein cows PMID: 16388132
results indicate that serine 84 is crucial for the activity of bGH despite this site being distal to the receptor binding surfaces PMID: 16514176
GH dramatically inhibits the expression of IGFBP-5, and GH along with IGF-I enhanced the phosphorylation of Akt through the reduction of IGF binding protein (IGFBP)-5. PMID: 16698222
insulin-regulated resensitization of GH signaling may be necessary to reset the complete response to GH after a normal, physiologic pulse of GH PMID: 16717097
A missense mutation within the GH1 gene is associated with body fat distribution. PMID: 16734678
GH, IGF-I and IGF-IBP3 regulated the growth and development traits in different growth period. PMID: 16870578
Characterization of single nucleotide polymorphisms in GH1 in relation to production traits. PMID: 16879354
nitration of JAK2 may act as an inhibitory counterpart to phosphorylation activation, reflecting a very localized break on the progression of GH signal transduction processes spanning JAK-STAT-AKT interactions PMID: 17510232
Gh(deficient (dr/dr) rats, which are nearly refractory to mammary carcinogenesis, can be made vulnerable by restoring GH and IGF-I. Advanced rat mammary cancers are dependent on GH and/or IGF-I for their survival. PMID: 17584969
Transmission ratio distortion at the growth hormone gene (GH1) in bovine preimplantation embryos PMID: 17948248
overexpression of growth hormone is associated with kidney diseases PMID: 18097769
Peak-shaped responses were observed for concentrations of LH and GH, and the peaks were observed at 27+/-3 and 75+/-9 min, respectively, after injection, only in heifers injected with kisspeptin-10. PMID: 18252956
940 bGH haplotypes were compared with respect to six carcass traits: carcass weight, longissimus muscle area, rib thickness, subcutaneous fat thickness, beef marbling score and beef colour. PMID: 18254825
Daytime and nighttime profiles of growth hormone(GH) secretion and the effect of a photic stimulation on nocturnal GH and melatonin secretion were investigated in Holstein steers. PMID: 18375661
GH expression (bGH) and its receptor binding (GHR-/-) had profound and divergent effects on muscle phenotype. PMID: 18499495
Age-related changes in body composition of bovine growth hormone transgenic mice. PMID: 18948397
AluI polymorphism of the growth hormone gene had no effect on the interval from calving to first ovulation and could not be directly related to differences in milk yield and to the extent of body condition loss during the first month after calving. PMID: 19111333
Chronic GH excess increased FA metabolism in the young age, whereas its action was overwhelmed in elder ages likely by GH-independent mechanisms. PMID: 19342398