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  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Dog Complement 4(C4) ELISA kit
  • 中文名稱：
    犬補體蛋白4(C4)酶聯免疫試劑盒
  • 貨號：
    CSB-EQ027970DO
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 ng/mL-2000 ng/mL
  • 靈敏度：
    7.8 ng/mL
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    犬補體蛋白4(C4)酶聯免疫試劑盒（CSB-EQ027970DO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C4含量。試劑盒檢測范圍為31.25 ng/mL-2000 ng/mL，適用于動物模型研究、免疫系統功能評估、藥物干預效果觀測等科研場景，為犬類基礎醫學研究提供標準化工具；其濃度變化可為研究犬類免疫相關疾病機制、感染性疾病進程或自身免疫異常提供重要依據本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    C4
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:200Average %97
    Range %92-105
    1:400Average %96
    Range %92-99
    1:800Average %103
    Range %98-109
    1:1600Average %87
    Range %82-91
  • 回收率：
    The recovery of dog C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9488-98
    EDTA plasma (n=4)9087-95
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    20000.080 0.079 0.080
    10000.117 0.125 0.121
    5000.202 0.198 0.200
    2500.311 0.305 0.308
    1250.499 0.513 0.506
    62.50.927 0.933 0.930
    31.251.457 1.342 1.400
    02.559 2.438 2.499
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：狗

稀釋比：1:100

產品評價： 我用犬補體蛋白C4酶聯免疫試劑盒檢測犬血清中C4含量，結果良好，標曲線性良好，復孔RSD小于15%,稀釋后樣本濃度均在線性范圍內。三個指標試劑盒標曲是采用LOG-LOG模擬計算的。

By 房老師



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