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  4. Fish growth hormone,GH ELISA Kit
  • 中文名稱：
    魚生長激素(GH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12121Fh
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    312.5 pg/mL-5000 pg/mL
  • 靈敏度：
    312.5 pg/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚生長激素(GH)酶聯(lián)免疫試劑盒（CSB-E12121Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH含量。GH（生長激素）是重要內(nèi)分泌靶點。背景上，其在人體生長、代謝調(diào)節(jié)等方面意義重大。研究機制方面，主要通過與生長激素受體結(jié)合，激活下游信號通路，影響細(xì)胞增殖、分化與代謝，相關(guān)研究有助于了解生長發(fā)育及治療相關(guān)疾病。試劑盒檢測范圍為312.5 pg/mL-5000 pg/mL，適用于實驗室研究魚類生長相關(guān)基因表達(dá)、營養(yǎng)調(diào)控實驗或激素分泌動態(tài)監(jiān)測，為科研人員提供穩(wěn)定可靠的數(shù)據(jù)支持，助力水產(chǎn)生物學(xué)及比較內(nèi)分泌學(xué)領(lǐng)域的深入研究；其定量分析對水產(chǎn)養(yǎng)殖優(yōu)化、魚類生理機制研究及環(huán)境因子對生長的影響評估具有重要意義。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 關(guān)聯(lián)名：
    gh1 ELISA Kit; Somatotropin-1 ELISA Kit; Growth hormone I ELISA Kit; Somatotropin I ELISA Kit
  • 縮寫：
    GH
  • Uniprot No.：
  • 種屬：
    Fish
  • 反應(yīng)時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Developmental Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-98  
    1:2 Average % 92  
    Range % 88-96  
    1:4 Average % 102  
    Range % 96-106  
    1:8 Average % 96  
    Range % 92-101  
  • 回收率：
    The recovery of fish GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 92-98  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    5000 0.271 0.265 0.268    
    2500 0.526 0.514 0.520    
    1250 0.887 0.863 0.875    
    625 1.664 1.684 1.674    
    312.5 2.557 2.602 2.580    
  • 本試劑盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-Fish GH antibody.
    • Standard (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • HRP-avidin (1 x 6 ml) --Catalyze the TMB to elicit a chromogenic reaction.
    • Biotin-conjugated GH (1 x 6 ml) --Bind to the precoated antibody and act as the detection antigen.
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
    • Substrate A (1x 7 ml) --Mix with Sbustrate B.
    • Substrate B (1x 7 ml) --Mix with Sbustrate A
    • Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：魚

稀釋比：沒有稀釋

產(chǎn)品評價： 我用CSB-E12121Fh試劑盒檢測不同性別羅非魚血清里GH激素含量，OD值普遍在0.2-0.8的范圍內(nèi)，效果很好。這個試劑盒步驟簡單，標(biāo)準(zhǔn)品也已經(jīng)稀釋好了，不用再自己配，非常方便。

By 吳老師

靶點詳情

  • 功能：
    Growth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family


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