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Human Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

  • 中文名稱:
    人Ⅰ型前膠原羧基端肽(PⅠCP)酶聯免疫試劑盒
  • 貨號:
    CSB-E08079h
  • 樣本類型:
    serum, plasma, urine
  • 檢測范圍:
    62.5 pg/mL-4000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人Ⅰ型前膠原羧基端肽(PⅠCP)酶聯免疫試劑盒(CSB-E08079h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、尿液樣本中的PⅠCP含量。人Ⅰ型前膠原羧基端肽(PⅠCP)是反映骨代謝的重要指標,其水平變化與骨吸收和骨形成密切相關。研究顯示,PⅠCP在骨質疏松、骨折愈合等疾病診斷和治療監測中具有重要作用。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,常用于骨轉化研究、纖維化疾病模型評估及組織修復機制探索等科研領域。適用于骨代謝相關細胞實驗、動物模型研究,以及肝纖維化、肺纖維化等疾病機制研究,尿液樣本的兼容性為無創性研究提供了便利。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 關聯名:
    Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
  • 縮寫:
    PⅠCP
  • 種屬:
    Homo sapiens (Human)
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 99-101  
    1:200 Average % 91  
    Range % 85-97  
    1:400 Average % 87  
    Range % 82-94  
    1:800 Average % 95  
    Range % 90-100  
  • 回收率:
    The recovery of human PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 81-93  
    EDTA plasma (n=4) 100 93-106  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.356 2.397 2.377 2.184  
    2000 2.004 2.049 2.027 1.834  
    1000 1.633 1.674 1.654 1.461  
    500 1.221 1.265 1.243 1.050  
    250 0.869 0.855 0.862 0.669  
    125 0.578 0.598 0.588 0.395  
    62.5 0.367 0.398 0.383 0.190  
    0 0.196 0.190 0.193    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型:細胞培養上清液

樣品信息:人

稀釋比:1:50

產品評價: 我使用CSB-E08079h試劑盒檢測了人血清樣本中的Ⅰ型前膠原羧基端原肽(PⅠCP)。標準曲線OD值范圍在0.190-2.377之間(濃度范圍62.5 pg/mL-4000 pg/mL),梯度良好,線性關系清晰。樣本檢測的OD值大致落在標準曲線的中段區間。試劑盒的精密度高,批內CV%<8%,批間CV%<10%,重復性很好。整個操作流程順暢,靈敏度達到15.6 pg/mL,能夠準確反映樣本中PⅠCP的含量變化,是一款性能穩定可靠的ELISA試劑盒,值得推薦。

By 侯老師



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