哎呀哎呀在线观看视频|狼狼鲁色在线视频播放|天天躁日日躁狠狠躁欧美老妇|波斯女人1977满天星|九阳真经未满十八岁能学吗|被老外做的都肿了|7tv影院

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Human Pantetheinase(VNN1) ELISA kit

Human Pantetheinase(VNN1) ELISA kit

  • 中文名稱：
    人泛酰巰基乙胺酶(VNN1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL025883HU
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人泛酰巰基乙胺酶(VNN1)酶聯免疫試劑盒（CSB-EL025883HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VNN1含量。VNN1 即Vanin - 1，是一種在多種組織表達的泛酰巰基乙胺酶。其與氧化應激、炎癥等生理病理過程關聯。研究機制上，它通過調節半胱胺水平影響細胞內氧化還原狀態，在代謝性疾病、腫瘤等研究中受關注，為相關疾病防治提供潛在靶點。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于基礎科研領域對VNN1功能的探索性研究，例如在代謝疾病模型或炎癥模型中分析VNN1的動態表達規律，評估藥物干預或基因調控對VNN1活性的影響，或通過多組學聯合分析挖掘其作為潛在生物標志物的價值；為疾病機制研究及藥物開發提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 關聯名：
    HDLCQ8 ELISA Kit; High density lipoprotein cholesterol level quantitative trait locus 8, included ELISA Kit; Pantetheinase ELISA Kit; Pantetheine hydrolase ELISA Kit; Tiff66 ELISA Kit; V-1 ELISA Kit; Vanin 1 ELISA Kit; Vanin-1 ELISA Kit; Vannin 1 ELISA Kit; Vascular non inflammatory molecule 1 ELISA Kit; Vascular non-inflammatory molecule 1 ELISA Kit; VNN 1 ELISA Kit; VNN1 ELISA Kit; VNN1_HUMAN ELISA Kit
  • 縮寫：
    VNN1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human pantetheinase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-104
    1:2Average %84
    Range %80-88
    1:4Average %90
    Range %85-94
    1:8Average %100
    Range %95-104
  • 回收率：
    The recovery of human pantetheinase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8880-92
    EDTA plasma (n=4)9490-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    50001.984 2.117 2.051 1.949
    25001.402 1.326 1.364 1.262
    12500.763 0.802 0.783 0.681
    6250.446 0.475 0.461 0.359
    312.50.275 0.284 0.280 0.178
    156.20.201 0.193 0.197 0.095
    780.164 0.157 0.161 0.059
    00.106 0.098 0.102
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.
  • 基因功能參考文獻：
    1. findings suggest that the metabolic pathway catalyzed by VNN1 pantetheinase plays a suppressive role in IAV infection in the respiratory tract, especially in severe conditions under hypercytokinemia PMID: 28576495
    2. Data show that G protein-coupled receptor, family C, group 5, member A protein (GPRC5A) regulates oxidative stress through vanin 1 protein (VNN1). PMID: 28316092
    3. These data suggest that urinary vanin-1 is an early predictive biomarker for decline in eGFR in patients with urothelial carcinoma after dosing of cisplatin PMID: 27317936
    4. serum pantetheinas, encoded by the VNN1 gene, level regulates erythrocyte life span and modulates the risk of developing complicated malaria. PMID: 26343328
    5. VNN1 contributes to corticosteroid responsiveness, and changes in VNN1 nasal epithelial mRNA expression and VNN1 promoter methylation might be clinically useful biomarkers of treatment response in asthmatic children. PMID: 25910714
    6. Our study provides strong biological evidence for the association of the identified SNP with BP and suggests that vanin-1 misfolding and degradation are the underlying molecular mechanism. PMID: 25233454
    7. show that hepatic vanin-1 is under extremely sensitive regulation by PPARalpha and that plasma vanin activity could serve as a readout of changes in PPARalpha activity in human subjects PMID: 24751833
    8. The X-ray crystal structure of human vanin 1 at 2.25 A resolution is presented, which is the first reported structure from the vanin family, as well as a crystal structure of vanin 1 bound to a specific inhibitor. PMID: 25478849
    9. Serum vanin-1 levels may be low in the end-stage renal failure and transiently increase after transplant owing to transient renal function deterioration, which does not lead to elevation of serum creatinine levels in renal transplant patients. PMID: 24702142
    10. Our results suggest that vanin-1 can distinguish between chronic responders and non-responders ITP patients as well as between newly diagnosed ITP patients and healthy controls. PMID: 23534352
    11. Alternative Splicing in VNN1 gene is associated with colorectal cancer. PMID: 24687856
    12. Single-nucleotide polymorphisms in the Regulatory Regions of the VNN1 Gene Are Associated with inflammatory bowel diseases. PMID: 23949622
    13. Microparticle internalization required the interaction of the ectoenzyme Vanin-1 (VNN1), an abundant surface protein on the microparticles, with lipid raft domains of endothelial cells. PMID: 24106341
    14. It isthe enzyme that recycles pantothenic acid (vitamin B5) generated during coenzyme A breakdown and actively involved in the progression of inflammatory reactions by generating cysteamine. PMID: 23978960
    15. Liver contributes to Vanin-1 secretion in serum and PPARalpha is a limiting factor in serum Vnn1 production. PMID: 24140347
    16. data do not support a major role for the Vanin 1 T26I variant in determining blood pressure level and incident ischemic events PMID: 21550219
    17. Our data suggest that VNN1 gene expression and the G-137T variant are associated with HDL-C levels in Mexican children, particularly in prepubertal girls. PMID: 23185446
    18. Urinary vanin-1, an ectoenzyme pantetheinase, distinguished diabetic patients with macroalbuminuria from those with normal albuminuria. PMID: 21544065
    19. Vanin-1 is a specific and sensitive biomarker for renal tubular injury induced by organic solvents. PMID: 21907259
    20. Overexpression of VNN1, an oxidative stress sensor in epithelial cells, was most strongly associated with progression to chronic immune thrombocytopenia PMID: 21325602
    21. The combination of VNN1 and MMP9 may be used as a novel blood biomarker panel for the discrimination of pancreatic cancer-associated diabetes from type 2 diabetes. PMID: 20571492
    22. Pantetheinase (Vnn1) is critical for the host susceptibility to malaria. PMID: 17312006
    23. missense SNP rs2272996 (or N131S) in the VNN1 gene was significantly associated with hypertension in African Americans and the association was replicated in Mexican Americans; a non-significant opposite association was observed in European Americans PMID: 18043751
    24. vanin-1 and vanin-3 are induced at the mRNA and protein level by psoriasis-associated proinflammatory cytokines (Th17/Th1) but not by Th2 cytokines. PMID: 19322213

    顯示更多

    收起更多

  • 亞細胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Carbon-nitrogen hydrolase superfamily, BTD/VNN family
  • 組織特異性：
    Widely expressed with higher expression in spleen, kidney and blood. Overexpressed in lesional psoriatic skin.
  • 數據庫鏈接：

    HGNC: 12705

    OMIM: 603570

    KEGG: hsa:8876

    STRING: 9606.ENSP00000356905

    UniGene: Hs.12114



×
生命科學計算平臺