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Your Good Partner in Biology Research

  • 中文名稱(chēng):
    人可溶性彈性蛋白片段(sELAF)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E14110h
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    3.12 ng/mL-200 ng/mL
  • 靈敏度:
    0.78 ng/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷(xiāo):
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人可溶性彈性蛋白片段(sELAF)酶聯(lián)免疫試劑盒(CSB-E14110h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的sELAF含量。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL,適用于基礎(chǔ)科研中探究彈性蛋白代謝異常相關(guān)疾病模型、藥物干預(yù)效果評(píng)估或生物標(biāo)記物篩選,例如通過(guò)分析心血管疾病患者血清或肺纖維化動(dòng)物模型組織勻漿中的sELAF水平,揭示其與疾病進(jìn)展的關(guān)聯(lián)性。支持科研人員高效開(kāi)展彈性蛋白代謝調(diào)控機(jī)制研究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    sELAF
  • 種屬:
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human sELAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %90-100
    1:2Average %101
    Range %93-105
    1:4Average %95
    Range %88-98
    1:8Average %91
    Range %87-94
  • 回收率:
    The recovery of human sELAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9991-102
    EDTA plasma (n=4)8782-91
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.821 2.777 2.799 2.676
    1001.886 1.826 1.856 1.733
    501.236 1.222 1.229 1.106
    250.719 0.739 0.729 0.606
    12.50.383 0.374 0.379 0.256
    6.250.276 0.284 0.280 0.157
    3.120.190 0.185 0.188 0.065
    00.121 0.125 0.123
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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