Human enkephalin,ENK ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載一鍵復(fù)制產(chǎn)品信息

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人腦啡肽(ENK)酶聯(lián)免疫試劑盒（CSB-E09117h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的ENK含量。人腦啡肽（ENK）是一種內(nèi)源性阿片肽，具有鎮(zhèn)痛、調(diào)節(jié)情緒和行為等功能。其研究機(jī)制涉及與腦內(nèi)阿片受體結(jié)合，通過(guò)抑制疼痛信號(hào)傳遞和調(diào)節(jié)神經(jīng)遞質(zhì)來(lái)發(fā)揮作用。靶點(diǎn)背景研究顯示，ENK主要作用于μ、δ、κ和痛敏肽受體，其生物學(xué)功能廣泛，與多種生理和病理過(guò)程相關(guān)。試劑盒檢測(cè)范圍為0.05 ng/mL-10 ng/mL，適用于科研領(lǐng)域?qū)ι窠?jīng)遞質(zhì)調(diào)控機(jī)制的探索；可應(yīng)用于神經(jīng)生物學(xué)、藥理學(xué)研究中ENK動(dòng)態(tài)變化的檢測(cè)，或用于評(píng)估藥物干預(yù)、行為學(xué)模型對(duì)腦啡肽系統(tǒng)的影響，為探索神經(jīng)系統(tǒng)功能提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

ENK
種屬：

Homo sapiens (Human)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-99
1:2	Average %	93
	Range %	87-100
1:4	Average %	93
	Range %	85-99
1:8	Average %	90
	Range %	82-102

回收率：

The recovery of human ENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	97	93-105

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.