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  4. Human telomerase,TE ELISA Kit
  • 中文名稱：
    人端粒酶(TE)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08021h
  • 樣本類型：
    serum, cell culture supernates, urine, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人端粒酶(TE)酶聯(lián)免疫試劑盒（CSB-E08021h）為雙抗夾心法ELISA試劑盒，定量檢測血清、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的TE含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，科研人員可通過分析疾病模型動物或體外培養(yǎng)細胞中的端粒酶表達動態(tài)，探索腫瘤發(fā)生機制、評估抗衰老藥物對端粒酶活性的調(diào)控作用，或利用尿液樣本開展無創(chuàng)性研究。其寬泛的樣本兼容性為組織水平研究、細胞實驗數(shù)據(jù)驗證及生物標志物篩選提供了可靠工具，適用于基礎醫(yī)學、藥理學及分子生物學領域的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 關聯(lián)名：
    N/A
  • 縮寫：
    TE
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-96
    1:2Average %99
    Range %91-105
    1:4Average %94
    Range %92-98
    1:8Average %89
    Range %86-100
  • 回收率：
    The recovery of human TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8980-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.630 2.501 2.566 2.464
    102.161 2.062 2.112 2.010
    51.602 1.541 1.572 1.470
    2.50.931 0.898 0.915 0.813
    1.250.532 0.501 0.517 0.415
    0.6250.322 0.314 0.318 0.216
    0.3120.241 0.227 0.234 0.132
    00.105 0.099 0.102 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：蛋白（重組/純化） 細胞裂解后的胞漿蛋白

樣品信息：人

稀釋比：其他 1:2

產(chǎn)品評價： 我用CSB-E08021h測量癌細胞裂解液中的端粒酶含量，稀釋一倍，OD在0.3左右。標曲正常，端粒酶定量也符合文獻報道。使用方便，如果可以的話盒子做小一點，更好保存。

By 黃老師

相關產(chǎn)品

相關問答

■ 常見問題解答
Q:

We are interesting in knowing the composition and the origin of the standard as well as capture and biotin antibody in this kit.

A:
Thanks for your inquiry.
Coated antibody: Mouse monoclonal antibody
Detect antibody: Goat polyclonal antibody
Standard: It is prepared with a special method and sorry we cannot provide the detailed information. Hope you could understand.


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