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  4. Mouse Angiotensin converting enzyme,ACE ELISA Kit

Mouse Angiotensin converting enzyme,ACE ELISA Kit

  • 中文名稱：
    小鼠血管緊張素轉化酶(ACE)酶聯免疫試劑盒
  • 貨號：
    CSB-E04492m
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠血管緊張素轉化酶(ACE)酶聯免疫試劑盒（CSB-E04492m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ACE含量。ACE即血管緊張素轉換酶，在腎素-血管緊張素-醛固酮系統（RAAS）中起關鍵作用。它能催化血管緊張素 I 轉化為血管緊張素 II，引起血管收縮、血壓升高等。目前針對 ACE 研發了眾多降壓藥物，通過抑制其活性發揮治療作用。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，靈敏度為0.39 ng/mL。評估高血壓模型小鼠的循環系統ACE水平變化、研究藥物干預對組織ACE表達的影響，或通過不同體液/組織樣本的橫向對比揭示ACE的分布特征；為心血管疾病機制研究、藥效學評價及分子調控網絡分析提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 關聯名：
    Ace ELISA kit; Dcp1Angiotensin-converting enzyme ELISA kit; ACE ELISA kit; EC 3.2.1.- ELISA kit; EC 3.4.15.1 ELISA kit; Dipeptidyl carboxypeptidase I ELISA kit; Kininase II ELISA kit; CD antigen CD143) [Cleaved into: Angiotensin-converting enzyme ELISA kit; soluble form] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 95  
    Range % 84-101  
    1:10 Average % 96  
    Range % 86-103  
    1:20 Average % 96  
    Range % 92-100  
    1:40 Average % 95  
    Range % 87-101  
  • 回收率：
    The recovery of mouse ACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-103  
    EDTA plasma (n=4) 92 85-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    100 2.153 2.141 2.147 1.971  
    50 1.542 1.596 1.569 1.393  
    25 1.117 1.104 1.111 0.935  
    12.5 0.703 0.688 0.696 0.520  
    6.25 0.489 0.474 0.482 0.306  
    3.12 0.322 0.318 0.320 0.144  
    1.56 0.259 0.266 0.263 0.087  
    0 0.172 0.180 0.176 　  
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:20

產品評價： 我用CSB-E04492m分別檢測了造模及治療前后小鼠血清中ACE的水平含量，發現治療之后小鼠血清中ACE水平下降了。第一次接觸ELISA，有很多操作細節不清楚，聯系了他們售后，非常耐心地一一作了解答了，幫忙處理處理數據，方便好多。

By 申老師

靶點詳情

  • 最新研究進展：
        血管緊張素轉化酶（Angiotensin Converting Enzyme，ACE）是一種重要的酶類分子，具有調節血管收縮、水鹽代謝、免疫反應等多種生理作用。近年來，對ACE的研究逐漸深入，以下是最新的研究進展：
        ACE在疾病中的作用機制得到了深入研究。例如，ACE與高血壓、冠心病、肺動脈高壓等疾病的關聯性研究已經得到了廣泛關注。研究人員發現，ACE可以調節血管緊張素的生成，進而影響血管的收縮和擴張，從而對心血管系統的功能產生影響。
        ACE的代謝和信號轉導途徑研究取得了新進展。研究人員已經發現多種ACE受體，并且探索了它們與ACE的結合機制。同時，ACE代謝途徑中的關鍵酶類的作用機制也得到了更深入的研究。
         ACE在藥物研發中的應用也取得了一定進展。例如，ACE抑制劑已經成為治療高血壓和心血管疾病的一類重要藥物。此外，研究人員還通過對ACE的結構和功能的研究，探索了一些新型ACE抑制劑的設計和合成方法。
        ACE與免疫系統的關系也引起了研究人員的關注。研究發現，ACE不僅能夠調節血管緊張素的生成，還能夠調節多種細胞因子的生成，從而影響免疫反應的進行。
     
  • 功能：
    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.
  • 基因功能參考文獻：
    1. ACE expression/phosphorylation in the bone-marrow niche interface negatively regulates G-CSF-induced signaling and hematopoietic progenitor cell mobilization. PMID: 29549541
    2. first evidence indicating the causation between ACE DD or B2R+9bp genotype and the increased risk for diabetic nephropathy, broadening our horizon about the role of genetic modulators in this disease. PMID: 28390948
    3. a small increase in angiotensin-converting enzyme activity in diabetic animals leads to greater impairment of autonomic function, as demonstrated by increased sympathetic modulation and reduced cardiac vagal modulation along with increased renal expression of Ang II. PMID: 30088535
    4. Resveratrol upregulated ACE2 and inhibited abdominal aortic aneurysm growth in a mouse model. PMID: 28935757
    5. In mice with renal injury induced by L-NAME pretreatment, renal tubular epithelial ACE is essential for renal angiotensin II accumulation and salt-sensitive hypertension PMID: 27988209
    6. Studied ACE role in peptide processing and for MHC class II antigen presentation; found ACE level effects efficiency of antigen presentation, and overexpression or inhibition of ACE alters the CD4(+) T-cell and antibody response. PMID: 28394320
    7. ACE enhances the oxidative response and bactericidal activity of neutrophils. PMID: 28515091
    8. this study shows that angiotensin-converting enzyme inhibitor captopril rescues mice from endotoxin-induced lethal hepatitis PMID: 27879415
    9. Smooth muscle cell-derived ACE contributes to atherosclerosis, independent of circulating ACE activity and blood pressure. PMID: 27055902
    10. Intestinal ACE shedding is increased by DSS-induced intestinal inflammation and parallels local corticosterone production. ACE product angiotensin II stimulates corticosterone formation in healthy intestine. PMID: 26401072
    11. Renin angiostensin system contributes to 20-HETE-mediated microvascular remodeling in hypertension and that 20-HETE-driven microvascular remodeling independent of blood pressure elevation does not fully rely on ACE activity in the vascular endothelium. PMID: 25924878
    12. angiotensin-converting enzyme has an essential role in hypertension induced by nitric oxide synthesis inhibition PMID: 25012170
    13. Suggest that the ACE2-ACE imbalance plays an important role in the pathogenesis of severe acute pancreatitis and that pancreatic ACE2 is an important factor in determining the severity of SAP. PMID: 24414175
    14. Show that tissue-specific targets are critical for the effects of miR-143/145 on smooth muscle differentiation and that angiotensin converting enzyme is one such target. PMID: 25273883
    15. Tissue-specific expression of transgenic secreted ACE in vasculature can restore normal kidney functions, but not blood pressure, of Ace-/- mice. PMID: 24475296
    16. ACE-null mice produce large numbers of myeloid-derived suppressor cells during chronic inflammation. PMID: 24614194
    17. Myelomonocytic expression of catalytically active ACE, prevents cognitive decline in a murine model of Alzheimer disease. PMID: 24487585
    18. ACE mediates angiotensin I-induced atherosclerosis, and ACE expression in leukocytes modestly contributes to atherosclerotic development in hypercholesterolemic mice. PMID: 23846498
    19. TEX101 is a unique specific substrate for ACE that is essential for the production of fertile mouse spermatozoa PMID: 23633567
    20. renal ACE activity is required to increase local Ang II, to stimulate sodium transport in loop of Henle and the distal nephron, and to induce hypertension PMID: 23619363
    21. deregulation of ACE and ACE2 plays an important role in tourniquet-induced renal injury PMID: 22580272
    22. When Ang I and CMP48/80 were co-administered, AT(1B) receptor expression was increased, MCP-4 was found surrounding the vessel wall PMID: 21622682
    23. shapes the MHC class I peptide repertoire for antigen presentation to T-cells PMID: 21964607
    24. Mature adipocytes modulate the expression profile of macrophages by releasing lipid mediators that increase ACE expression. PMID: 21116821
    25. results demonstrate a previous unrecognized significant role for ACE in myelopoiesis and imply new perspectives for manipulating myeloid cell expansion and maturation. PMID: 21148418
    26. Leptin regulates ACE activity in mice PMID: 20614101
    27. Angiotensin-converting enzyme overexpression in mouse myelomonocytic cells augments resistance to Listeria and methicillin-resistant Staphylococcus aureus PMID: 20937811
    28. A modest genetic increase in ACE impairs myocardial tolerance to ischemia. ACE level plays a critical role in cardiac ischemia, through both kinin and angiotensin mediated mechanisms. PMID: 20667972
    29. data provide direct evidence that small genetic disturbances in ACE levels per se have an influence on haemodynamic, cardiac mass and autonomic nervous system responses in mice under pathological perturbation PMID: 19930431
    30. angiotensin-converting enzyme has a role in improving autonomic dysregulation in ob/ob mice PMID: 20012594
    31. data suggest that the two catalytic domains of mACE do not function independently but that the signal transduction is influenced by negative cooperativity of the two catalytic domains PMID: 20030584
    32. There is a switch from ACE-dependent to serine protease-dependent ANG II formation in the type II diabetic kidney. PMID: 19846569
    33. data suggest that ACE is essential to ANG II formation in the vascular space PMID: 12003835
    34. urine-concentrating defect in mice with absence of tissue ACE is associated with downregulation of key urea, salt, and water transport proteins PMID: 12167603
    35. Chymase may play a role in heart remodeling by increasing Ang II formation and activating MMP-9, and the regulation of collagen I gene expression. PMID: 12359984
    36. gender differences in expression of ACE in the heart PMID: 12384478
    37. results demonstrated that the rodent germinal angiotensin converting enzyme is released from the testicular sperm membrane when sperm enter the epididymis PMID: 12444051
    38. There is no any association between angiotensin converting enzyme polymorphism and Alzheimer's disease. PMID: 12459519
    39. Substantial evidence is provided for a major role for angiotensin converting enzyme in a murine allergic contact dermatitis model of neurogenic infolammation. PMID: 12646655
    40. Expression of ACE, either in renal proximal tubules or in vasculature, is sufficient for maintaining normal kidney functions, but for maintaining blood pressure, ACE must be expressed in vascular endothelial cells. PMID: 12777443
    41. the presence of the C-terminal ACE catalytic domain is sufficient to maintain a functional renin-angiotensin system PMID: 14757757
    42. ACE is the predominant pathway of angiotensin II formation in blood and tissues of mice and plays a major role in bradykinin (1-9) metabolism in blood and, to a lesser extent, in kidney and heart. PMID: 14769811
    43. t-ACE contributes to arterial structure/remodeling & hyperplastic carotid inward remodeling from blood flow cessation. It is not needed for outward hypertrophic & subsequent inward hypotrophic remodeling of the uterine artery during/after pregnancy. PMID: 15031129
    44. Aldosterone upregulated macrophage ACE mRNA expression and activity. The selective effect of aldosterone on tissue ACE, versus circulating ACE, further demonstrates differences in the regulation pathways of tissue and circulating ACE. PMID: 15123520
    45. ACE Tyr phosphorylation, probably in the endoplasmic reticulum, enhances the rate of its cleavage secretion at the plasma membrane using a regulatory pathway that may involve p38 MAP kinase PMID: 15252021
    46. Mice with cardiac-restricted angiotensin-converting enzyme have atrial enlargement, cardiac arrhythmia, and sudden death. PMID: 15331425
    47. Ptn has the potential to critically regulate the downstream activities of angiotensin II, through the regulation of its synthesis by ACE and its receptor mediated functions through regulation of both the AT1 and AT2 receptors PMID: 15485659
    48. renal tissue angiotensin converting enzyme is an important contributor to tubuloglomerular feedback PMID: 15494545
    49. ACE is associated with body weight and peri-epididymal fat accumulation PMID: 15522949
    50. ACE signaling may underlie the increase in COX-2 and prostacyclin levels in patients treated with ACE inhibitors. PMID: 15569856

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  • 亞細胞定位：
    [Angiotensin-converting enzyme, soluble form]: Secreted.; Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Detected in both cell membrane and cytoplasm in neurons.
  • 蛋白家族：
    Peptidase M2 family
  • 組織特異性：
    Testis-specific isoform is expressed in spermatocytes, adult testis.
  • 數據庫鏈接：

    KEGG: mmu:11421

    STRING: 10090.ENSMUSP00000001963

    UniGene: Mm.754



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