Mouse Apolipoprotein B48 (apo-B48) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書下載一鍵復(fù)制產(chǎn)品信息

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠載脂蛋白B48(apo-B48)酶聯(lián)免疫試劑盒（CSB-E16506m）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的apo-B48含量。apo-B48是重要的血脂相關(guān)靶點(diǎn)。其背景在于它是乳糜微粒的主要載脂蛋白，在脂質(zhì)代謝中起關(guān)鍵作用。研究機(jī)制主要圍繞調(diào)控其合成、分泌等環(huán)節(jié)，以降低血漿中富含甘油三酯脂蛋白水平，為高脂血癥等疾病治療提供新方向。試劑盒檢測(cè)范圍為62.5 ng/mL-4000 ng/mL，適用于研究脂代謝相關(guān)動(dòng)物模型中apo - B48的動(dòng)態(tài)變化，例如高脂飲食誘導(dǎo)的肥胖小鼠、腸道功能缺陷模型或基因編輯動(dòng)物；為探究脂蛋白代謝調(diào)控機(jī)制、藥物干預(yù)效果評(píng)估等基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

apo-B48
種屬：

Mus musculus (Mouse)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse apo-B48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	96
	Range %	92-104
1:400	Average %	93
	Range %	89-97
1:800	Average %	87
	Range %	84-92
1:1600	Average %	87
	Range %	82-93

回收率：

The recovery of mouse apo-B48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	98	94-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.