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  4. Mouse Growth/differentiation factor 11(GDF11) ELISA kit

Mouse Growth/differentiation factor 11(GDF11) ELISA kit

  • 中文名稱：
    小鼠生長分化因子11(GDF11)酶聯免疫試劑盒
  • 貨號：
    CSB-EL009344MO
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.68 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠生長分化因子11(GDF11)酶聯免疫試劑盒（CSB-EL009344MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GDF11含量。GDF11是轉化生長因子-β超家族成員。其在胚胎發育等過程發揮重要作用。研究機制上，參與調節組織器官的生長、發育與修復，在神經、肌肉等系統有相關作用，近年在衰老研究中受關注，研究其對延緩衰老進程的潛在作用。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于科研場景中探究GDF11在發育生物學、再生醫學及衰老相關疾病模型中的表達動態，例如通過檢測不同發育階段或病理狀態下血清、腦組織、肌肉等樣本的蛋白水平變化，揭示其在組織穩態維持或再生修復中的作用機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 關聯名：
    Gdf11 ELISA Kit; Bmp11Growth/differentiation factor 11 ELISA Kit; GDF-11 ELISA Kit; Bone morphogenetic protein 11 ELISA Kit; BMP-11 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GDF11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %85-97
    1:2Average %89
    Range %84-95
    1:4Average %86
    Range %82-93
    1:8Average %100
    Range %95-107
  • 回收率：
    The recovery of mouse GDF11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-102
    EDTA plasma (n=4)10298-107
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12002.531 2.598 2.565 2.418
    6001.937 1.967 1.952 1.805
    3001.280 1.291 1.286 1.139
    1500.710 0.763 0.737 0.590
    750.430 0.465 0.448 0.301
    37.50.294 0.284 0.289 0.142
    18.750.225 0.231 0.228 0.081
    00.146 0.148 0.147 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues. It is required for proper vertebral patterning and orofacial development. Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3.
  • 基因功能參考文獻：
    1. critical regulator of kidney fibrosis and tubular function PMID: 29731246
    2. study indicates that GDF11/8 in the kidney decreases with age and that GDF11 can increase tubular cell dedifferentiation and proliferation as well as improve tubular regeneration after acute kidney injury (AKI) in old mice. PMID: 27703192
    3. Our results showed that GDF11 inhibited osteoblastic differentiation of bone marrow mesenchymal stem cells in vitro and had no effect on osteoclast differentiation or bone resorption PMID: 27395058
    4. In conclusion, our findings show that GDF11 expression declines with age and the protective effects of ultrasound-targeted microbubble destruction-mediated delivery of GDF11 on the aged ischemic heart provide support for the classification of GDF11 as an anti-aging factor PMID: 28004242
    5. we found that myostatin forms a complex with LTBP4 and that overexpression of LTBP4 led to a decrease in myostatin levels. LTBP4 also interacted with TGFbeta and GDF11, a protein highly related to myostatin. These data identify LTBP4 as a multi-TGFbeta family ligand binding protein with the capacity to modify muscle disease through overexpression PMID: 27148972
    6. Circulating levels of GDF11/8 declines with age in mice (and sheep, horses and rats). PMID: 26489925
    7. Data show that circulating myostatin levels decreased with age and estimates of growth differentiation factor 11 (GDF11) levels using myostatin null mice indicate that they were almost 500 times lower than those for myostatin. PMID: 26372181
    8. Two new studies demonstrate that GDF11 this "factor of youth" rejuvenates stem cells found in the skeletal muscle and brain of aged mice. PMID: 25112712
    9. Therefore, we postulate that GDF-11DeltaEx1 may act as a long non-coding RNA to regulate the transcription of canonical GDF-11 and/or other genes in skeletal muscle and other tissues PMID: 24378996
    10. Expression of GDF11, a cytokine which blocks terminal erythroid maturation, was increased in erthyroblasts of thalassemic mice. PMID: 24658077
    11. GDF11 inhibited erythroid maturation in mice in vivo and ex vivo. Expression of GDF11 in erythroid precursors decreased progressively with maturation, suggesting an inhibitory role for GDF11 in late-stage erythroid differentiation. PMID: 24658078
    12. Gdf11 stimulates expression of a Hoxd11/lacZ transgene in the mouse embryo tailbud. PMID: 24016758
    13. GASP-1 and GASP-2 are important modulators of GDF-11 and MSTN activity in vivo. PMID: 24019467
    14. Gdf11 signaling is a major coordinator of the trunk-to-tail transition during mouse development PMID: 23763947
    15. Using modified aptamer-based proteomics, study identified the TGF-beta superfamily member GDF11 as a circulating factor in young mice that declines with age. PMID: 23663781
    16. These results suggest that over-expression of BMP11 propeptide stimulates bone formation by increasing osteoblast cell functions. PMID: 22093826
    17. PCSK5 and GDF11 expression during hindgut morphogenesis could be key factors for normal anorectal development, which can be disturbed by the administration of an overdose of all-trans retinoic acid, leading to anorectal malformations. PMID: 21480163
    18. Significant effects of GDF11 propeptide transgene on vertebral formation. PMID: 21049546
    19. Gdf11 secreted by newly born neurons in the developing spinal cord facilitates the temporal progression of neurogenesis by acting as a positive feedback signal on the progenitor cells to promote cell cycle exit and decrease proliferation ability. PMID: 21248112
    20. GDF11 signaling regulates development of caudal vertebrae and is involved in specification of axial vertebrae in part by maintaining Cyp26a1 expression. PMID: 20801112
    21. Activin type IIB(ActRIIB) and its subfamily receptor, Activin type IIA (ActRIIA), cooperatively mediate the Gdf11 signal in patterning the axial vertebrae PMID: 12414726
    22. GDF11 plays a role in autoregulation of neurogenesis PMID: 12546816
    23. Gdf11 may be important in directing the initial outgrowth of the ureteric bud from the Wolffian duct by controlling the expression of Gdnf in the metanephric mesenchyme.Gdf11 also regulates kidney organogenesis PMID: 12729564
    24. GDF11 regulates the production and maturation of islet progenitor cells in pancreas development. PMID: 15548585
    25. GDF11 controls the numbers of retinal ganglion cells (RGC's) as well as amacrine and photoreceptor cells that form during development; it controls duration of expression of Math5, a gene that confers competence for RGC genesis, in progenitor cells PMID: 15976303
    26. GDF11 forms a noncovalent latent complex with its SPC-cleaved prodomain and that this latent complex is activated via cleavage at a single specific site by members of the developmentally important BMP1/Tolloid family of metalloproteinases. PMID: 15988002
    27. Gdf11 has an important function in determining Hox gene expression domains and rostrocaudal identity in the caudal spinal cord. PMID: 16790475
    28. Growth differentiation factor 11 signals through the transforming growth factor-beta receptor ALK5 to regionalize the anterior-posterior axis. PMID: 16845371
    29. Gdf11 transcripts are expressed in embryonic pancreas epithelium before the secondary transition but decrease rapidly afterward. PMID: 16964608
    30. the selectivity of PC5/6 for Gdf11 essentially resides in the presence of a P1' Asn in the RSRR downward arrowN cleavage motif PMID: 18378898
    31. We propose that Pcsk5, at least in part via GDF11, coordinately regulates caudal Hox paralogs, to control anteroposterior patterning, nephrogenesis, skeletal, and anorectal development. PMID: 18519639
    32. Functional validation of activins and bone morphogenetic protein 11 as candidate novel muscle mass regulators. PMID: 18927237
    33. defects in development of the cerebral hemispheres in Foxg1(-/-) mice are not rescued by mutations in Gdf11, nor is Gdf11 expressed at high levels within these structures PMID: 19297409
    34. Study shows that Mstn-/- Gdf11-/- mice have more extensive homeotic transformations of the axial skeleton than Gdf11-/- mice in addition to skeletal defects not seen in single mutants such as extra forelimbs. PMID: 19298661

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 組織特異性：
    Highly expressed in the developing limb bud, initially detected in the distal mesenchyme, and later localizing to regions around the developing bones. Is also expressed in adult dental pulp and brain.
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