Mouse High density lipoprotein cholesterol, HDL-C ELISA kit

產(chǎn)品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠高密度脂蛋白膽固醇檢測試劑盒（CSB-E12874m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HDL-C含量。HDL-C（高密度脂蛋白膽固醇）是一種被稱為“好”的膽固醇，有助于清除血液中的多余膽固醇，降低心臟病風(fēng)險。研究顯示，高HDL-C水平與較低的冠心病風(fēng)險相關(guān)，其作用機(jī)制包括促進(jìn)膽固醇逆向轉(zhuǎn)運(yùn)和抗氧化等。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，適用于心血管疾病模型、肥胖或糖尿病研究中脂代謝動態(tài)監(jiān)測、藥物干預(yù)效果評估等科研場景。通過檢測血清、血漿樣本可系統(tǒng)性評估循環(huán)系統(tǒng)中HDL-C水平，而組織勻漿檢測則有助于探索特定器官（如肝臟、脂肪組織）的局部脂質(zhì)代謝調(diào)控機(jī)制，為代謝性疾病基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

HDL-C
種屬：

Mus musculus (Mouse)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	88
	Range %	84-92
1:2000	Average %	91
	Range %	85-97
1:4000	Average %	99
	Range %	95-104
1:8000	Average %	92
	Range %	88-96

回收率：

The recovery of mouse HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	97	91-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.