Mouse Immunoglobulin A, IgA ELISA Kit

產(chǎn)品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠免疫球蛋白A(IgA)酶聯(lián)免疫試劑盒（CSB-E07986m）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的IgA含量。IgA是一種免疫球蛋白，主要存在于黏膜表面，具有抗菌和抗病毒作用。IgA腎病是一種以IgA沉積在腎小球系膜區(qū)為特征的腎小球疾病，其發(fā)病機(jī)制復(fù)雜，涉及遺傳、環(huán)境因素和免疫反應(yīng)等多重打擊假說。目前，針對(duì)IgA腎病的研究主要集中在靶向B細(xì)胞免疫通路、補(bǔ)體系統(tǒng)等靶點(diǎn)，以開發(fā)更有效的治療藥物。試劑盒檢測(cè)范圍為25 ng/mL-1600 ng/mL，腸道菌群研究、呼吸道感染模型評(píng)估、免疫調(diào)節(jié)劑藥效分析、小鼠感染性疾病模型、自身免疫疾病研究、過敏反應(yīng)實(shí)驗(yàn)中黏膜免疫水平的動(dòng)態(tài)監(jiān)測(cè)、免疫機(jī)制探索、生物標(biāo)志物分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IgA
種屬：

Mus musculus (Mouse)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	93
	Range %	88-95
1:10000	Average %	95
	Range %	90-99
1:20000	Average %	89
	Range %	85-94
1:40000	Average %	97
	Range %	91-102

回收率：

The recovery of mouse IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	102	98-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.