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Your Good Partner in Biology Research

  • 中文名稱:
    小鼠循環(huán)免疫復(fù)合物(CIC)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E16553m
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    15.6 ng/mL-1000 ng/mL
  • 靈敏度:
    3.9 ng/mL
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠循環(huán)免疫復(fù)合物(CIC)酶聯(lián)免疫試劑盒(CSB-E16553m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的CIC含量。小鼠循環(huán)免疫復(fù)合物(CIC)是體內(nèi)抗原與抗體結(jié)合形成的復(fù)合物,多數(shù)情況下對機(jī)體有益,但在某些情況下,如免疫復(fù)合物沉積,可能引起組織損傷和疾病。CIC的研究涉及其形成機(jī)制、臨床意義以及與疾病如類風(fēng)濕關(guān)節(jié)炎的關(guān)系。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL,適用于小鼠自身免疫疾病模型構(gòu)建、感染性疾病研究中免疫復(fù)合物沉積機(jī)制探索,或藥物干預(yù)后免疫調(diào)節(jié)效應(yīng)的評估,也可用于免疫學(xué)、病理學(xué)等基礎(chǔ)研究中對樣本中 CIC 水平進(jìn)行檢測以探究免疫調(diào)節(jié)機(jī)制或評估疾病模型狀態(tài)。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 關(guān)聯(lián)名:
    N/A
  • 縮寫:
    CIC
  • 種屬:
    Mus musculus (Mouse)
  • 反應(yīng)時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:20Average %95
    Range %89-103
    1:40Average %90
    Range %81-100
    1:80Average %91
    Range %86-99
    1:160Average %95
    Range %91-104
  • 回收率:
    The recovery of mouse CIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-102
    EDTA plasma (n=4)9690-104
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.107 2.053 2.080 1.923
    5001.593 1.575 1.584 1.427
    2501.097 1.023 1.060 0.903
    1250.635 0.611 0.623 0.466
    62.50.449 0.426 0.438 0.281
    31.20.302 0.283 0.293 0.136
    15.60.226 0.217 0.222 0.065
    00.152 0.162 0.157
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型:血清

樣品信息:小鼠

稀釋比:1:100

產(chǎn)品評價: 我用小鼠循環(huán)免疫復(fù)合物酶聯(lián)免疫試劑盒檢測小鼠血清CIC濃度,采用四參數(shù)擬合標(biāo)曲,r2=1.000,標(biāo)曲準(zhǔn)確度在-4.73~5.61%范圍內(nèi),推薦稀釋倍數(shù)合理,檢測結(jié)果很好。

By 房老師

相關(guān)問答

 常見問題解答
Q:

這個ELISA試劑盒能夠與C1q結(jié)合嗎?

A:
測量CIC有特異性抗原和非特異性抗原兩種方法。 C1q結(jié)合ELISA屬于后者,但與CSB-E16553m使用的方法有所不同。


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