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Rat Vasoactive Intestinal Peptide,VIP ELISA Kit

  • 中文名稱:
    大鼠血管活性腸肽(VIP)酶聯免疫試劑盒
  • 貨號:
    CSB-E08355r
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 pg/mL-100 pg/mL
  • 靈敏度:
    0.39 pg/mL
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠血管活性腸肽(VIP)酶聯免疫試劑盒(CSB-E08355r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的VIP含量。VIP即血管活性腸肽,具有廣泛生物學活性。它參與調節心血管、消化等系統功能。在研究機制上,VIP通過與特定受體結合,激活細胞內信號通路,影響多種生理和病理過程,在神經保護、抗炎等方面展現潛在治療價值。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL,適用于神經生物學、消化系統疾病模型研究,可分析大鼠外周循環或組織局部VIP表達水平,為探究胃腸動力障礙、炎癥調節機制及神經 - 免疫交互作用提供數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %97
    Range %93-101
    1:4Average %100
    Range %97-103
    1:8Average %86
    Range %82-92
  • 回收率:
    The recovery of rat VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9086-94
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.384 2.506 2.445 2.324
    501.732 1.813 1.773 1.652
    251.112 1.100 1.106 0.985
    12.50.615 0.626 0.621 0.500
    6.250.386 0.397 0.392 0.271
    3.120.297 0.286 0.292 0.171
    1.560.196 0.193 0.195 0.074
    00.121 0.120 0.121  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 3 個評價

樣品類型:血清 ,組織勻漿液 腸

樣品信息:大鼠

稀釋比:其他 1:5

產品評價: 我用CSB-E08355r檢測大鼠組織勻漿液和大鼠血清的血管活性腸肽(VIP)濃度,標準曲線的OD值在1.0左右,樣本的OD值在0.1-1.0之間,結果均在線性范圍之內。

By 林老師

樣品類型:血清

樣品信息:大鼠

稀釋比:其他 1:5

產品評價: 我用該試劑盒(貨號CSB-E08355r)檢測大鼠血清樣本,樣本按1:5比例稀釋后操作,標準曲線的OD值線性關系良好,相關系數達0.996,檢測結果重復性極佳。試劑盒操作步驟清晰,試劑活性穩定,2-8℃儲存下未出現性能衰減,對大鼠VIP的檢測特異性強,無明顯交叉反應,完全滿足科研實驗的定量需求,是一款性價比很高的ELISA試劑盒。

By 婁老師

樣品類型:血清

樣品信息:大鼠

稀釋比:其他 1:2

產品評價: 我用1:2稀釋倍數稀釋大鼠血清,得到的結果符合預期,非常符合預期。試劑盒試劑穩定,測出結果合理,沒有弄虛作假

By 劉老師



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