Rat cytochrome C oxidase(COX) ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠細(xì)胞色素C氧化酶(COX)酶聯(lián)免疫試劑盒（CSB-E14281r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的COX含量。COX即環(huán)氧合酶，是催化花生四烯酸轉(zhuǎn)化為前列腺素的限速酶。它有COX-1和COX-2兩種同工酶，COX-1為結(jié)構(gòu)型，參與維持機(jī)體生理功能；COX-2為誘導(dǎo)型，在炎癥等病理狀態(tài)下表達(dá)增加。其研究機(jī)制多圍繞對這兩種同工酶的作用開展。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域，如探究心血管疾病、神經(jīng)退行性疾病或代謝綜合征等模型中COX的活性變化，也可用于評估藥物或環(huán)境因素對線粒體能量代謝通路的影響，為細(xì)胞呼吸機(jī)制研究、病理模型構(gòu)建及藥理實(shí)驗(yàn)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
關(guān)聯(lián)名：

N/A
縮寫：

COX
種屬：

Rattus norvegicus (Rat)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat COX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	82-99
1:2	Average %	94
	Range %	86-100
1:4	Average %	93
	Range %	85-100
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of rat COX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-100
EDTA plasma (n=4)	96	89-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.