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  4. Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

  • 中文名稱：
    大鼠角化細胞內(nèi)分泌因子(KAF)/雙調(diào)蛋白(AR)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07299r
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.25 pg/mL-80 pg/mL
  • 靈敏度：
    0.31 pg/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠角化細胞內(nèi)分泌因子(KAF)/雙調(diào)蛋白(AR)酶聯(lián)免疫試劑盒（CSB-E07299r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AREG含量。AREG（Amphiregulin）是一種表皮生長因子家族成員，主要在腫瘤組織中高表達，與腫瘤的增殖、侵襲和轉(zhuǎn)移密切相關(guān)。研究顯示，AREG基因的高表達與患者年齡及腫瘤細胞分化程度密切相關(guān)，其高表達可能與啟動子區(qū)去甲基化有關(guān)。此外，AREG在多種惡性腫瘤中異常活躍，如結(jié)直腸癌、非小細胞肺癌等，其過表達會加速腫瘤生長和轉(zhuǎn)移。試劑盒檢測范圍為1.25 pg/mL-80 pg/mL，該產(chǎn)品為科研領(lǐng)域提供可靠工具，可用于研究皮膚疾病模型、上皮損傷修復機制、慢性炎癥反應(yīng)或腫瘤微環(huán)境中KAF/AR的動態(tài)變化，特別適用于動物實驗中的分子機制探索及生物標志物分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 關(guān)聯(lián)名：
    Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應(yīng)時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-98
    1:2Average %95
    Range %91-99
    1:4Average %86
    Range %80-92
    1:8Average %96
    Range %92-102
  • 回收率：
    The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10498-108
    EDTA plasma (n=4)9792-106
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    802.538 2.616 2.577 2.413
    401.939 2.059 1.999 1.835
    201.364 1.386 1.375 1.211
    100.882 0.870 0.876 0.712
    50.549 0.541 0.545 0.381
    2.50.341 0.356 0.349 0.185
    1.250.253 0.258 0.256 0.092
    00.168 0.160 0.164 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
  • 基因功能參考文獻：
    1. Report increased AREG transcript and protein levels in the lungs of rodents with DA-induced bronchiolitis obliterans. PMID: 24816162
    2. estrogen and progeterone increased cell proliferation in normal gland and mammary cancers through the increased production of amphiregulin PMID: 21258428
    3. may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats PMID: 15291759
    4. the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues PMID: 15509566
    5. findings identify Amphiregulin as a factor for resistance of glioma cells to delta9-Tetrahydrocannabinol induced apoptosis PMID: 19229996

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  • 亞細胞定位：
    Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Amphiregulin family
  • 數(shù)據(jù)庫鏈接：


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