Rat oxyntomodulin ELISA Kit

產品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠胃泌酸調節素酶聯免疫試劑盒（CSB-E12949r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的OXM含量。OXM（胰高血糖素樣肽 -1/胰高血糖素雙受體激動劑）具有獨特背景。它涉及調節能量代謝、血糖的生理機制。研究表明，OXM可作用于相應受體，促進胰島素分泌、抑制胰高血糖素分泌、延緩胃排空，有望用于肥胖、糖尿病等疾病的治療。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于大鼠模型中OXM表達水平與胃腸功能、代謝調控的相關性研究，可應用于藥物干預、飲食調控或病理狀態下OXM動態變化的科研分析。樣本處理簡便，標準曲線覆蓋生理及病理濃度范圍，滿足基礎研究中血清、血漿等液態樣本及胃腸組織勻漿的檢測需求，為消化系統與代謝調控領域的分子機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

OXM
種屬：

Rattus norvegicus (Rat)
反應時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	96
	Range %	90-102
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of rat oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-100
EDTA plasma (n=4)	99	95-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.