Rat thrombomodulin,TM ELISA Kit
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中文名稱:大鼠血栓調(diào)節(jié)蛋白(TM)酶聯(lián)免疫試劑盒
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貨號:CSB-E07939r
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:1.56 ng/mL-100 ng/mL
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靈敏度:0.39 ng/mL
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規(guī)格:96T/48T
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價格:¥3900/¥2500
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促銷:
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠血栓調(diào)節(jié)蛋白(TM)酶聯(lián)免疫試劑盒(CSB-E07939r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的TM含量。大鼠血栓調(diào)節(jié)蛋白(TM)是一種由內(nèi)皮細(xì)胞表達(dá)的凝血酶結(jié)合蛋白,具有抗凝、抑制血小板聚集和纖溶等作用。其表達(dá)與深靜脈血栓形成(DVT)的病理過程密切相關(guān),可能成為DVT早期診斷和治療的潛在靶點(diǎn)。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL,科研人員可將其應(yīng)用于心血管疾病模型、藥物作用機(jī)制、內(nèi)皮細(xì)胞功能研究等領(lǐng)域,例如評估病理狀態(tài)下TM的表達(dá)變化或探究干預(yù)措施對凝血調(diào)控的影響,為體外實(shí)驗(yàn)提供可靠的定量分析工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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關(guān)聯(lián)名:N/A
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縮寫:TM
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種屬:Rattus norvegicus (Rat)
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反應(yīng)時間:1-5h
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上樣量:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 99 Range % 87-95 1:2 Average % 94 Range % 89-97 1:4 Average % 99 Range % 94-102 1:8 Average % 95 Range % 91-99 -
回收率:
The recovery of rat TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 91-97 EDTA plasma (n=4) 95 92-97 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 100 2.888 2.824 2.856 2.689 50 2.259 2.234 2.247 2.080 25 1.432 1.421 1.427 1.260 12.5 0.777 0.789 0.783 0.616 6.25 0.476 0.454 0.465 0.298 3.12 0.328 0.335 0.332 0.165 1.56 0.246 0.237 0.242 0.075 0 0.169 0.165 0.167 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- The liver sinusoidal endothelial cell damage in rats caused by heatstroke Xingqin Zhang.et al,European Journal of Inflammation,2018
- Benefits of anti Wei W.et al,Mol Med Rep. ,2017
- Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock Hayakawa M.et al,Shock.,2015
- Electrical Vagus Nerve Stimulation Attenuates Systemic Inflammation and Improves Survival in a Rat Heatstroke Model Yamakawa K et al,PLoS One,2013
產(chǎn)品評價
樣品類型:血清
樣品信息:大鼠
稀釋比:沒有稀釋
產(chǎn)品評價: 本圖為血清檢測二次擬合曲線,r=0.989,擬合度優(yōu)異,數(shù)據(jù)點(diǎn)與曲線貼合度高。隨X軸數(shù)值增加,Y軸響應(yīng)值呈顯著遞增趨勢,檢測線性關(guān)系良好,說明試劑盒在檢測范圍內(nèi)靈敏度與重復(fù)性達(dá)標(biāo),實(shí)驗(yàn)數(shù)據(jù)可靠,可支持后續(xù)定量分析。
By 王老師
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