CUSABIO貨號(hào)：CSB-MA275281 PDHA1單克隆抗體是針對(duì)丙酮酸脫氫酶復(fù)合體E1α亞基（PDHA1）研發(fā)的高特異性科研試劑，該靶點(diǎn)在糖代謝通路中發(fā)揮核心作用，通過催化丙酮酸轉(zhuǎn)化為乙酰輔酶A調(diào)控細(xì)胞能量供應(yīng)，其表達(dá)異常與腫瘤發(fā)生、代謝性疾病密切相關(guān)。本產(chǎn)品經(jīng)嚴(yán)格驗(yàn)證可識(shí)別人類、小鼠、大鼠、犬及豬等多種物種的PDHA1蛋白，適用于Western Blot、ELISA及免疫細(xì)胞化學(xué)等實(shí)驗(yàn)平臺(tái)，在細(xì)胞能量代謝機(jī)制研究、癌癥相關(guān)信號(hào)通路解析以及糖尿病等代謝疾病模型構(gòu)建中具有重要應(yīng)用價(jià)值。該抗體采用雜交瘤技術(shù)制備，具備優(yōu)異的批間穩(wěn)定性和抗原結(jié)合特異性，能夠精準(zhǔn)識(shí)別天然及變性狀態(tài)下的靶蛋白，為研究PDHA1在生理病理過程中的分子調(diào)控網(wǎng)絡(luò)提供可靠工具，適用于基礎(chǔ)科研領(lǐng)域的蛋白表達(dá)分析、亞細(xì)胞定位研究及分子互作實(shí)驗(yàn)設(shè)計(jì)。關(guān)鍵詞：PDHA1單克隆抗體、丙酮酸脫氫酶、糖代謝研究、Western Blot、免疫細(xì)胞化學(xué)、能量代謝調(diào)控。

pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial PDHE1-A type I pyruvate dehydrogenase complex, E1-alpha polypeptide 1

Mouse

Recombinant fragment , corresponding to amino acids 131aa-389aa of Human PDHA1 (C-terminus).

Homo sapiens (Human)

Monoclonal

IgG1

Antigen Affinity Purified

12C9

It differs from different batches. Please contact us to confirm it.

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

Application	Recommended Dilution
WB	1:500-1:5000
ICC	1:50-1:200

ELISA Protocol
Western Blotting (WB) Protocol

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.