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  4. AGPAT5 Antibody
  • 中文名稱：
    AGPAT5兔多克隆抗體
  • 貨號：
    CSB-PA001453LA01HU
  • 規格：
    ￥2024
  • 圖片：
    • Western Blot
      Positive WB detected in: B16F10 whole cell lysate(30μg), A2780 whole cell lysate(30μg), Hela whole cell lysate(30μg), HL-60 whole cell lysate(30μg), THP-1 whole cell lysate(30μg), K562 whole cell lysate(20μg),SY5Y whole cell lysate(30μg),293 whole cell lysate(30μg),Mouse Brain tissue lysate(20μg)
      All lanes: AGPAT5 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 43 kDa
      Observed band size: 43 kDa
      Exposure time: 60s
    • IHC image of CSB-PA001453LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA001453LA01HU diluted at 1:100 and staining in paraffin-embedded human Liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA001453LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) AGPAT5 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    AGPAT5; 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon; 1-acylglycerol-3-phosphate O-acyltransferase 5; 1-AGP acyltransferase 5; 1-AGPAT 5; Lysophosphatidic acid acyltransferase epsilon; LPAAT-epsilon
  • 宿主：
    Rabbit
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    Synthesized peptide derived from internal of human AGPAT5
  • 免疫原種屬：
    Homo sapiens (Human)
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen affinity purification
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C15:0-C20:4 at the sn-1 position using C18:1-CoA as the acyl donor. Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA. Activity toward lysophosphatidylglycerol not detectable.
  • 基因功能參考文獻：
    1. enzymatic properties, tissue distribution, and subcellular localization of human AGPAT3 and AGPAT5 PMID: 21173190
    2. This publication describes the molecular cloning, tissue distribution, and enzyme characterization of the mouse homolog, mAGPAT5. PMID: 15367102
  • 亞細胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus envelope. Mitochondrion.
  • 蛋白家族：
    1-acyl-sn-glycerol-3-phosphate acyltransferase family
  • 組織特異性：
    Widely expressed.
  • 數據庫鏈接：

    HGNC: 20886

    OMIM: 614796

    KEGG: hsa:55326

    STRING: 9606.ENSP00000285518

    UniGene: Hs.624002



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