IHC image of CSB-PA883440LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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貨期:
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用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Minus-end microtubule-dependent motor protein. Acts as a negative regulator of centrosome separation required to prevent premature centrosome separation during interphase. Required to maintain a centered nucleus to ensure that the spindle is stably oriented at the onset of mitosis. May also act as a negative regulator of amino acid starvation-induced autophagy.
基因功能參考文獻:
The activity of Kif25 during interphase is required to maintain a centred nucleus to ensure the spindle is stably oriented at the onset of mitosis. PMID: 28263957
亞細胞定位:
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
蛋白家族:
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family