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  4. ST8SIA2 Antibody
  • 中文名稱：
    ST8SIA2兔多克隆抗體
  • 貨號：
    CSB-PA852840ESR2HU
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: SY5Y whole cell lysate(30μg), 293 whole cell lysate(30μg), U251 whole cell lysate(30μg), JK whole cell lysate(30μg), A549 whole cell lysate(30μg), HepG2 whole cell lysate(30μg),Mouse Heart tissue lysate(30μg),Mouse Brain tissue lysate(30μg),Mouse Kidney tissue lysate(30μg)
      All lanes: ST8SIA2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 43 kDa
      Observed band size: 43 kDa
      Exposure time: 10s
    • IHC image of CSB-PA852840ESR2HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA852840ESR2HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA852840ESR2HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Immunofluorescence staining of A549 cell with CSB-PA852840ESR2HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of HepG2 cell with CSB-PA852840ESR2HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) ST8SIA2 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    ST8SIA2
  • 別名：
    ST8SIA2 antibody; SIAT8B antibody; STX antibody; Alpha-2,8-sialyltransferase 8B antibody; EC 2.4.99.- antibody; Sialyltransferase 8B antibody; SIAT8-B antibody; Sialyltransferase St8Sia II antibody; ST8SiaII antibody; Sialyltransferase X antibody; STX antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    Synthesized peptide derived from part region of human protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:20-1:100
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression.
  • 基因功能參考文獻：
    1. The ST8SIA2 risk haplotype copy number did not show any differential effects on white matter in schizophrenia. PMID: 29353880
    2. impairment of the regulated expression of ST8SIA2 and the resulting downstream effects on gene products by these two intronic single nucleotide polymorphism contribute to the development of these psychiatric disorders. PMID: 27565727
    3. ST8SiaII role in the invasiveness and metastasis of small cell lung cancer cells PMID: 28004110
    4. the relationships between psychiatric disorders and polySia and/or ST8SIA2 PMID: 27105834
    5. Data indicate a possible role of STX polysialyltransferase (ST8SIA2) in the common susceptibility of schizophrenia and bipolar I disorder (BD-I). PMID: 26418860
    6. Our study showed interesting sex-specific associations between ST8SIAII and schizophrenia. PMID: 24070986
    7. the role of ST8SIA2 sequence variation affecting polysialic acid-NCAM formation in 48 Caucasian cases with bipolar disorder PMID: 24651862
    8. translational modification of the neural cell adhesion molecule NCAM and the polysialyltransferase ST8SiaII in mammalian semen involves polysialic acid PMID: 23671285
    9. Expression of ST8SIA2 in omental fat at baseline was significantly associated with weight loss after Roux-en-Y gastric bypass. PMID: 23643386
    10. that variation the ST8SIA2 gene is associated with increased risk to mental illness PMID: 22693595
    11. NCAM and polySia are expressed and developmentally regulated in chick corneas. Both membrane-associated and soluble NCAM isoforms are expressed in chick corneas. PMID: 22281821
    12. ST8SIA2 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
    13. STX(G421A) shows a dramatic decrease in polySia synthetic activity on NCAM, whereas STX(C621G) does not; polySia shows a dopamine (DA) binding activity PMID: 21464126
    14. This study provided that ST8SIA2 are associated with Bipolar I in the Han Chinese population. PMID: 20386566
    15. SIAT8B has a role in neural development and sialic acid synthesis on NCAM PMID: 12138100
    16. SIAT8B gene is involved in schizophrenia in the Japanese. PMID: 16229822

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  • 亞細胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Glycosyltransferase 29 family
  • 組織特異性：
    Highly expressed in fetal brain, kidney and heart and to a much lesser extent in adult heart and thymus.
  • 數據庫鏈接：

    HGNC: 10870

    OMIM: 602546

    KEGG: hsa:8128

    STRING: 9606.ENSP00000268164

    UniGene: Hs.302341



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