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  4. nfsA Antibody
  • 中文名稱：
    nfsA Antibody
  • 貨號：
    CSB-PA323751XA01ENV
  • 規格：
    ￥880
  • 圖片：
    • Dot blot analysis of peptides corresponding to the immunogen was probed with nfsA Antibodyat a dilution of 1:1000. Blocking/Dilution buffer: 4% nonfat dry milk/PBS. Secondary antibody：Goat polyclonal to rabbit IgG at 1/40000 dilution Exposure time: 30s.
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Escherichia coli (strain K12) nfsA Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    nfsA
  • 別名：
    Oxygen-insensitive NADPH nitroreductase (EC 1.-.-.-) (Modulator of drug activity A) nfsA mda18 mdaA ybjB b0851 JW0835
  • 宿主：
    Rabbit
  • 反應種屬：
    Escherichia coli (strain K12)
  • 免疫原：
    Synthesized peptide derived from internal of Escherichia coli (strain K12) nfsA protein (226-240aa )
  • 免疫原種屬：
    Escherichia coli (strain K12)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.
  • 基因功能參考文獻：
    1. Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin. PMID: 29753133
    2. reduction of quinones by NfsA is most consistent with a single-step (H(-)) hydride transfer mechanism PMID: 27986535
    3. Findings revealed that Phe42 is important for maintaining NfsA activity and structure. PMID: 23801116
    4. NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate. PMID: 22173483
  • 蛋白家族：
    Flavin oxidoreductase frp family
  • 數據庫鏈接：


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