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  4. secY Antibody
  • 中文名稱：
    secY Antibody
  • 貨號：
    CSB-PA314716XA01ENV
  • 規格：
    ￥880
  • 圖片：
    • Dot blot analysis of peptides corresponding to the immunogen was probed with secY Antibodyat a dilution of 1:1000. Blocking/Dilution buffer: 4% nonfat dry milk/PBS. Secondary antibody：Goat polyclonal to rabbit IgG at 1/40000 dilution Exposure time: 10s.
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Escherichia coli (strain K12) secY Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    secY
  • 別名：
    Protein translocase subunit SecY secY prlA b3300 JW3262
  • 宿主：
    Rabbit
  • 反應種屬：
    Escherichia coli (strain K12)
  • 免疫原：
    Synthesized peptide derived from internal of Escherichia coli (strain K12) secY protein (250-261aa )
  • 免疫原種屬：
    Escherichia coli (strain K12)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. SecY is required to insert newly synthesized SecY into the inner membrane. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true, overexpression also results in FtsH-mediated degradation of SecY.
  • 基因功能參考文獻：
    1. These data suggest that wild-type YidC residues (G355 and M471) participate in interactions, direct or indirect, with SecY. PMID: 24187090
    2. While translocation of loops 1 and 2 of TatC is strictly dependent on the Sec translocase and the YidC insertase, translocation of loop 3 does not depend on the translocase or insertase. PMID: 23058713
    3. SecY signal peptide is found to specifically bind SecY translocase in a saturable manner and at levels comparable to those that stimulate SecA ATPase activity. PMID: 15476412
    4. provide evidence for the direct interaction between FtsY and the SecY translocon PMID: 15815684
    5. SecY plug domain moves away from the center of the channel toward SecE during polypeptide translocation, and the translocation-enhancing prlA3 mutation and SecG subunit change the properties of channel gating. PMID: 16148946
    6. The data explain how conformational changes in SecA could be directly coupled to the previously proposed opening mechanism of the SecYEG channel. PMID: 16890955
    7. One such translocation complex, SecY/Sec61, is found in every cell, in either the plasma membrane of bacteria and archaea or the endoplasmic reticulum membrane of eukaryotes. PMID: 17077865
    8. the SecY plug contributes to the gating mechanism of the channel by maintaining the structure of the SecYEG complex in a compact closed state PMID: 17092931
    9. normal SecY function is required for the folding of membrane proteins after their insertion into the translocon PMID: 17242069
    10. study used purified E. coli components to address the mechanism of protein translocation; disulfide bridge crosslinking demonstrates that SecA transfers both the signal sequence and the mature region of a secreted substrate into a single SecY molecule PMID: 17418789
    11. The resting cell membrane channel forms a barrier for small molecules, with both the pore ring and the plug required for the seal; channel opening requires movement of the plug. PMID: 17531809
    12. Data suggest that SecY binds to the primary site on the ribosome and subsequently captures and translocates the nascent chain. PMID: 18158904

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  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SecY/SEC61-alpha family
  • 數據庫鏈接：


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