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  4. ACAT1 Recombinant Monoclonal Antibody

ACAT1 Recombinant Monoclonal Antibody

  • 中文名稱：
    ACAT1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA150416A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: COLO205 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), PC-3 whole cell lysate(30μg), K562 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), MCF7whole cell lysate(30μg), A2780 whole cell lysate(30μg)
      All lanes: ACAT1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 45 kDa
      Observed band size: 45 kDa
      Exposure time：1min
    • IHC image of CSB-RA150416A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA150416A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing HeLa cells stained with CSB-RA150416A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Acetyl-CoA acetyltransferase, mitochondrial (EC 2.3.1.9) (Acetoacetyl-CoA thiolase) (T2), ACAT1, ACAT MAT
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human ACAT1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    10H1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.
  • 基因功能參考文獻：
    1. High ACAT1 expression is associated with breast cancer. PMID: 30092298
    2. Insulin promotes progression of colon cancer by upregulation of ACAT1. PMID: 29793481
    3. ACAT1 exonic mutations that affect ESE sequences may result in aberrant splicing. This may affect the activity of mitochondrial acetoacetyl-CoA thiolase. PMID: 27748876
    4. compound heterozygous of ACAT1 gene mutations probably underlie the beta-ketothiolase deficiency in our patient PMID: 27264805
    5. Data indicate that acetyl-CoA acetyltransferase (ACAT1) and malate dehydrogenase (MDH2) are involved in various drug-resistance-forming mechanisms. PMID: 25639359
    6. the pyruvate dehydrogenase complex is regulated by Tyr phosphorylation of PDP1, which toggles recruitment between ACAT1 and SIRT3 PMID: 24486017
    7. these findings indicate that ACAT1 expression could serve as a potential prognostic marker in prostate cancer, specifically in differentiating indolent and aggressive forms of cancer. PMID: 24311408
    8. ACAT1 expression is substantially elevated in human castration-resistant metastatic prostate cancer tissues. PMID: 23443136
    9. Data show that the ketone body metabolizing enzymes BDH1, BDH2, OXCT1 and ACAT1 were expressed at the mRNA and protein level in all glioma cell lines. PMID: 21791085
    10. We herein identified a common mutation, R208X, in Vietnamese patients. We identified R208X homozygously in six patients and heterozygously in two patients among eight Vietnamese patients. PMID: 20156697
    11. the siblings with the same T2 gene mutations present different clinical severity of T2 deficiency PMID: 20046049
    12. Single base substitutions at the initiator codon produced wild-type T2 polypeptide to various degrees PMID: 12754704
    13. mild mutations (A132G, D339-V340insD) retained some residual T2 activity PMID: 15128923
    14. Cloning and sequencing long range PCR products revealed a 6.4kb deletion. Alu element-mediated unequal homologous recombination between an Alu-Sx in intron 1 and another Alu-Y in intron 4 appears to be responsible for this deletion. PMID: 16935016
    15. E252del is a temperature-sensitive K(m) mutant. PMID: 17236799
    16. Crystallographic and kinetic studies were made on mitochondrial acetyl-CoA thiolase: the importance of potassium and chloride ions were noted for its structure and function. PMID: 17371050
    17. A point mutation was responsible for the aberrant RNA splicing of the mitochondrial acetoacetyl-CoA thiolase gene. PMID: 18511318
    18. sequence recognition by the catalytic domain differs between hT2 and hT10 in that hT10 requires a pre-existing GalNAc residue while hT2 does not PMID: 18562306
    19. I/D polymorphism of ACE gene and AC AT1 gene influence the development of hypertension and Left Ventricular Hypertrophy in Hemodialysis patients. PMID: 19112833

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  • 相關疾病：
    3-ketothiolase deficiency (3KTD)
  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    Thiolase family
  • 數據庫鏈接：

    HGNC: 93

    OMIM: 203750

    KEGG: hsa:38

    STRING: 9606.ENSP00000265838

    UniGene: Hs.232375



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