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  4. AKAP14 Recombinant Monoclonal Antibody

AKAP14 Recombinant Monoclonal Antibody

  • 中文名稱：
    AKAP14 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA233959A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in:jurkat whole cell lysate(30μg), Mouse Kdiney whole cell lysate(30μg)
      All lanes: Lipoma preferred partner antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 23 kDa
      Observed band size: 23 kDa
      Exposure time：180s
    • Overlay Peak curve showing A549 cells stained with CSB-RA233959A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    AKAP14
  • 別名：
    A-kinase anchor protein 14 (AKAP-14) (A-kinase anchor protein 28 kDa) (AKAP 28) (Protein kinase A-anchoring protein 14) (PRKA14), AKAP14, AKAP28
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from Human AKAP14 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    21B9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
  • 基因功能參考文獻：
    1. AKAP28 likely plays a role in the signaling necessary for efficient modulation of ciliary beat frequency PMID: 12475942
  • 亞細胞定位：
    Cytoplasm.
  • 組織特異性：
    Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia.
  • 數據庫鏈接：

    HGNC: 24061

    OMIM: 300462

    KEGG: hsa:158798

    STRING: 9606.ENSP00000360485

    UniGene: Hs.592245



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