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  4. CPSF3 Recombinant Monoclonal Antibody

CPSF3 Recombinant Monoclonal Antibody

  • 中文名稱：
    CPSF3 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA072907A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HEK293 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), Hela whole cell lysate(30μg), Jurkat whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
      All lanes: CPSF73 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 77 kDa
      Observed band size: 77 kDa
      Exposure time：3min
    • IHC image of CSB-RA072907A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    CPSF3
  • 別名：
    Cleavage and polyadenylation specificity factor subunit 3 (EC 3.1.27.-) (Cleavage and polyadenylation specificity factor 73 kDa subunit) (CPSF 73 kDa subunit) (mRNA 3'-end-processing endonuclease CPSF-73), CPSF3, CPSF73
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from Human CPSF3 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    7B8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle. Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1. Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a. Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively.
  • 基因功能參考文獻(xiàn)：
    1. the molecular mechanism that recruits the CPSF73 endonuclease to histone pre-mRNAs PMID: 23071092
    2. The data support a model where Star-PAP binds to the pre-mRNA, recruits the CPSF complex to the 3'-end of pre-mRNA and then defines cleavage by CPSF 73 and subsequent polyadenylation of its target mRNAs. PMID: 21102410
    3. up-regulated by HIV-1 TAT protein; this could represent a novel mechanism by which HIV increases mRNA processing, causing an increase in both cell and viral gene expression. PMID: 15194760
    4. crystal structures of human CPSF-73 at 2.1 A resolution, complexed with zinc ions and a sulphate that might mimic the phosphate group of the substrate; CPSF-73 is the pre-mRNA 3'-end-processing endonuclease PMID: 17128255
    5. this study identifies CPSF-73 as an important regulatory protein that represses the basal transcriptional activity of the HIV-1 LTR promoter PMID: 17669424
    6. Conserved residues in CPSF-73 and CPSF2 are required for assembly of the endonuclease activity that cleaves histone pre-mRNAs. PMID: 18688255
    7. The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors. PMID: 19136632

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Metallo-beta-lactamase superfamily, RNA-metabolizing metallo-beta-lactamase-like family, CPSF3 subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2326

    OMIM: 606029

    KEGG: hsa:51692

    STRING: 9606.ENSP00000238112

    UniGene: Hs.515972



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