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  4. DPYSL5 Recombinant Monoclonal Antibody

DPYSL5 Recombinant Monoclonal Antibody

  • 中文名稱：
    DPYSL5 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA782941A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in:SH-SY5Y whole cell lysate(30μg), HEK293 whole cell lysate(30μg)
      All lanes: Lipoma preferred partner antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 61 kDa
      Observed band size: 61 kDa
      Exposure time：120s
    • IHC image of CSB-RA782941A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA782941A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA782941A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Dihydropyrimidinase-related protein 5 (DRP-5) (CRMP3-associated molecule) (CRAM) (Collapsin response mediator protein 5) (CRMP-5) (UNC33-like phosphoprotein 6) (ULIP-6), DPYSL5, CRMP5 ULIP6
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human DPYSL5 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    1E12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    May have a function in neuronal differentiation and/or axon growth.
  • 基因功能參考文獻：
    1. Study describes the relevance of DRP5 during osteosarcoma development. DRP5 was upregulated in osteosarcoma specimens and cell lines and shown to function via the downstream MMPs. Inhibition of DRP5 suppressed the growth of cancer cells in vitro and in vivo, and high expression levels of DRP5 were associated with poor prognosis in osteosarcoma patients. PMID: 28374915
    2. Our findings suggest that CRMP5 serves as a major mediator of Notch signaling and Akt activation by controlling the degradation of the Notch receptor, with implications for defining a biomarker signature in glioblastoma PMID: 26122847
    3. present study suggested that CRAM could be a clinical prognostic marker for patients with cervical cancer PMID: 24695578
    4. study elucidates a novel regulatory mechanism that utilizes CRMP5-induced mitophagy to orchestrate proper dendrite outgrowth and neuronal function. PMID: 24324268
    5. identified residues that are crucial for determining the preference for hetero-oligomer or homo-oligomer formation. In spite of being the CRMP family member most closely related to dihydropyrimidinase, CRMP-5 does not have amidohydrolase activity. PMID: 23373749
    6. New CRMP5 isoform present in the nucleus is associated with Glioma. PMID: 23298946
    7. CRMP-5-IgG defines a paraneoplastic ophthalmological entity of combined optic neuritis and retinitis with vitreous inflammatory cells. PMID: 12838519
    8. CRMP-5 autoimmune myelopathy and occult neoplasia are important considerations in patients with insidiously progressive myelopathy, especially with known cancer risk. PMID: 18306241
    9. We describe a patient with optic neuropathy and vitritis as the only clinical manifestations of paraneoplastic optic neuropathy secondary to lung cancer marked by an extremely high titer of CRMP-5 antibody. PMID: 18347453
    10. findings point at CRMP5 as a novel marker for distinguishing between highly aggressive neuroendocrine carcinoma and the other lung cancers. PMID: 18769332
    11. patients with CV2/CRMP5-Ab and thymoma developed myasthenic syndrome more frequently PMID: 18931014

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family
  • 數據庫鏈接：

    HGNC: 20637

    OMIM: 608383

    KEGG: hsa:56896

    STRING: 9606.ENSP00000288699

    UniGene: Hs.299315



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