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  4. EN1 Recombinant Monoclonal Antibody

EN1 Recombinant Monoclonal Antibody

  • 中文名稱：
    EN1 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA547193A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA547193A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing MCF-7? cells stained with CSB-RA547193A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    EN1
  • 別名：
    Homeobox protein engrailed-1 (Homeobox protein en-1) (Hu-En-1), EN1
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human EN1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    15G3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. Engrailed homeobox 1 was expressed specifically in normal eccrine glands and was expressed in most of the tumour cells of sweat gland neoplasms with eccrine gland differentiation. PMID: 29436004
    2. our finding suggested that the EN1 rs4144782 might contribute to the susceptibility of knee OA. PMID: 28430581
    3. FGFR Inhibitor Ameliorates Hypophosphatemia and Impaired Engrailed-1/Wnt Signaling in FGF2 High Molecular Weight Isoform PMID: 26762209
    4. low-frequency non-coding variant near a novel locus, EN1, with an effect on bone mineral density--which was also associated with a decreased risk of fracture PMID: 26367794
    5. EN1 is an activator of intrinsic inflammatory pathways associated with prosurvival in basal-like breast cancer. PMID: 24141779
    6. The current review describes the role of two such factors, Nurr1 and engrailed, in differentiation, maturation, and in normal physiological functions including acquisition of neurotransmitter identity. PMID: 24685177
    7. EN1 is a biologic predictor of poor prognosis in patients with salivary adenoid cystic carcinoma. PMID: 21800291
    8. Homeobox protein engrailed-1 protein regulates transcription of the utrophin gene. PMID: 21672318
    9. EN1 might be a negative regulatory factor for UTROPHIN. PMID: 21482524
    10. The result of this study found a strong association between the EN1 rs1438852 polymorphism and Parkinson's disease. PMID: 19345444
    11. En-1 exerts its repressive effect by a mechanism negatively controlling the level of beta-catenin. PMID: 16571670
    12. Single nucleotide polymorphisms genotyped in the EN1 gene were associated with schizophrenia symptoms PMID: 18698228
    13. Epigenetic suppression along 2q14.2 is common to most colorectal cancers and the presence of a methylated EN1 CpG island in stool DNA might be used as biomarker of neoplastic disease. PMID: 19384295
    14. Genetic variation in human engrailed 1 may influence antipsychotic response in schizophrenia. PMID: 18698228

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Engrailed homeobox family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 3342

    OMIM: 131290

    KEGG: hsa:2019

    STRING: 9606.ENSP00000295206

    UniGene: Hs.271977



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