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  4. HSF2 Recombinant Monoclonal Antibody

HSF2 Recombinant Monoclonal Antibody

  • 中文名稱：
    HSF2 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA214813A0HU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA214813A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA214813A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of HEK293 cell with CSB-RA214813A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA214813A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    HSF2
  • 別名：
    Heat shock factor protein 2 (HSF 2) (Heat shock transcription factor 2) (HSTF 2), HSF2, HSTF2
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human HSF2 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    3B2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:20-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • 基因功能參考文獻：
    1. Silencing ALG3 or HSF2 inhibited the proliferation, migration, and invasion abilities of MCF-7 cells. PMID: 29799832
    2. HSF2 is a direct target of miR202 with a binding site on 3'-UTR of HSF2 mRNA in esophageal squamous cell carcinoma cells. PMID: 28277193
    3. This study provides the first evidence for HSF2 acting as a suppressor of invasion in human malignancies. PMID: 26119944
    4. Presented are the crystal structures of the human HSF2 DNA-binding domain (DBD) bound to DNA. PMID: 26727490
    5. the findings of our studys suggest that HSF2 contributes to its own expression by forming a negative autoregulatory loop. PMID: 26260034
    6. HSF2 can induce histone activation.Set1/MLL complex is indispensable for the transcriptional ability of HSF2. PMID: 26478434
    7. HSF2 appears to be a potential novel molecular marker for ulcerative colitis PMID: 24533153
    8. the condensed chromatin of HSF2-deficient cells is accessible for HSF1 and RNA polymerase II, allowing stress-inducible Hsp expression. PMID: 25202032
    9. These results support a role for HSF2 in the pathogenesis of IA and further implicate this transcription factor as a potential therapeutic target. PMID: 23064888
    10. our findings suggest novel mechanisms of HSF2 regulation controlled by HSF4a. PMID: 21792930
    11. Data show that the PEST sequences of a short-lived protein called HSF2 interact with Cullin3, a subunit of a Cullin-RING E3 ubiquitin ligase, and that this interaction mediates the Cul3-dependent ubiquitination and degradation of HSF2. PMID: 19768582
    12. characterization of the hsf2 gene and its 5'-flanking region PMID: 11795475
    13. HSF2 influences to the formation of nuclear stress granules PMID: 12865437
    14. HSF1 has a role in transcriptional regulation of the human RANK ligand gene PMID: 14699143
    15. results show hsp70i bookmarking is mediated by HSF2 which binds this promoter in mitotic cells, recruits protein phosphatase 2A & interacts with the CAP-G subunit of condensin to promote dephosphorylation & inactivation of condensin complexes PMID: 15662014
    16. The results of these experiments indicate that the HSF2 region comprising amino acids 343-363 is important for A subunit interaction. PMID: 17688198
    17. During mitosis, HSF2 is bound to the HSE promoter elements of other heat shock genes, including hsp90 and hsp27, and the proto-oncogene c-fos. The presence of HSF2 is important for expression of these genes. PMID: 17915561
    18. MEL-18 bound to HSF2 inhibits its sumoylation by binding to and inhibiting the activity of UBC9 enzymes in the vicinity of HSF2. PMID: 18211895
    19. These results provide a potential mechanistic basis for the defective cytokinesis phenotype exhibited by HSF2-/- cells, as well as suggest a potential role for PRC1 in HSF2-mediated gene bookmarking. PMID: 18570919
    20. NMR study of the SUMOylated DNA binding domain of HSF2 indicates that the SUMO moiety is flexible with respect to the DNA binding domain and has neither a noncovalent interface with nor a structural effect on the domain PMID: 19017645
    21. HSF1-HSF2 heterotrimerization regulates stress-induced transcription PMID: 19129477

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation.
  • 蛋白家族：
    HSF family
  • 數據庫鏈接：

    HGNC: 5225

    OMIM: 140581

    KEGG: hsa:3298

    STRING: 9606.ENSP00000357440

    UniGene: Hs.158195



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