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  4. IVL Recombinant Monoclonal Antibody

IVL Recombinant Monoclonal Antibody

  • 中文名稱：
    IVL Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA296052A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HT29 whole cell lysate(30μg)
      All lanes: IVL antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 68 kDa
      Observed band size: 120 kDa
      Exposure time：3min
    • IHC image of CSB-RA296052A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA296052A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Involucrin, IVL
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human IVL protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    11G8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • 基因功能參考文獻：
    1. Together, these results suggest that GR modulates involucrin expression of keratinocytes by regulating the intracellular signaling network in a ligand-independent manner. PMID: 24515279
    2. over-expression of Nnat was evident along with involucrin and cytokeratin1 expression PMID: 24252747
    3. involucrin is up-regulated in psoriatic keratinocytes PMID: 23283814
    4. KLF4 is part of a multiprotein complex that interacts that the hINV promoter distal regulatory region to drive differentiation-dependent hINV gene expression in epidermis. PMID: 23599428
    5. Compared with the control, the level of expression of the IVL gene in NCTC 2544 cells was increased after 8 h of treatment with hyaluronic acid or Plantaricin A preparations. PMID: 22742591
    6. Therefore, together with trichohyalin, the cross-linking due to involucrin is also responsible for the mechanical resistance of the corneous trabeculae present among the empty spaces of the medulla of the human hair. PMID: 22197495
    7. The authors found that HPV 16 E6 but not E7 repressed IVL promoter activity in proliferating human foreskin keratinocyte cells. PMID: 22333115
    8. higher expression in the fetal skin than in adult skin PMID: 19701759
    9. expression of the epidermal differentiation complex genes filaggrin and involucrin was strongly decreased directly by IL-13. PMID: 20208004
    10. Transcriptional activation of the involucrin gene by calcium in keratinocytes. PMID: 9159190
    11. involucrin gene contains distinct regulatory elements that regulate expression during epidermal differentiation PMID: 11850802
    12. transactivation of involucrin by lens epithelium-derived growth factor (LEDGF) PMID: 12126624
    13. the AP-1 site and the vitamin D response element in the involucrin promoter play important roles in mediating the action of 1,25-dihydroxyvitamin D3 on involucrin expression PMID: 12445200
    14. p38 delta is the major p38 isoform driving suprabasal hINV gene expression PMID: 12810719
    15. role for AP1 transcription factors in the regulation of human corneal epithelial cell involucrin gene expression. PMID: 15037572
    16. human involucrin promoter activity is regulated by green tea polyphenol and curcumin via opposing effects on CCAAT/enhancer-binding protein function PMID: 15044435
    17. cascade regulates activator protein one, Sp1, and CCATT/enhancer-binding protein transcription factor DNA binding to two discrete involucrin promoter regions, the distal- and proximal-regulatory regions, to regulate involucrin gene expression. PMID: 15191537
    18. cholesterol depletion alters involucrin gene expression through activation of p38alpha/beta. PMID: 15304097
    19. YY-1 and c-Jun transcription factors participate in the repression of the human involucrin promoter. PMID: 15586248
    20. Involucrin expression of keratinocytes is suppressed by roxithromycin through direct inhibition of AP-1 and indirect inhibition of NF-kappaB. PMID: 16140218
    21. AP1, Sp1, and C/EBP transcription factors are required for appropriate differentiation-dependent involucrin expression. PMID: 16374477
    22. involucrin gene expression inhibition by apigenin is associated with a specific reduction in phosphorylation of protein kinase Cdelta Tyr311 PMID: 16982614
    23. Pyk2 regulation is associated with increased expression of Fra-1 and JunD, activator protein-1 transcription factors known to be required for involucrin expression. PMID: 17205062
    24. There is a major role for STAT5a in the differentiation of keratinocytes, where it contributes to involucrin expression by activating the PPARgamma signal. PMID: 17330131
    25. BAG-1L also potentiated transactivation of known vitamin-D-responsive gene promoters, osteocalcin and 24-hydroxylase, and enhanced VDR-dependent transcription and protein expression of the keratinocyte differentiation marker, involucrin. PMID: 17662274
    26. a large multiprotein complex, which includes Fra-1, p300, P/CAF, junD, junB, and Sp1 acts at the AP1-5 site to produce a synergistic increase in hINV gene expression PMID: 17882273
    27. These findings indicate that PKCdelta activity for activation of involucrin promoter requires Y(311) and a second tyrosine residue; however, any one of the several tyrosine residues can serve in the secondary role. PMID: 17943181
    28. PPAR gamma regulates involucrin expression by controlling the AP-1 signal and p38 activation in 1 alpha,25(OH)2D3-induced keratinocyte differentiation. PMID: 18077140
    29. The expression of loricrin and involucrin in invovled and uninvolved skin of patients with atopic dermatitis is reported. PMID: 18166499
    30. siRNA for PRKD1 resulted in reduction of mRNA levels of PKD1, altered cell phenotype and promotion of keratinocyte differentiation, demonstrated by increased expression of involucrin and keratin 10 mRNAs PMID: 18259765
    31. RT-PCR and western blot analysis revealed a delay in the expression of the differentiation markers K1, K10 and involucrin in HaCaT cells compared with normal human keratinocytes PMID: 18637039
    32. Infection by HPV may alter the differentiation status of the epidermis, leading to earlier involucrin expression PMID: 19515043

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  • 亞細胞定位：
    Cytoplasm. Note=Constituent of the scaffolding of the cornified envelope.
  • 蛋白家族：
    Involucrin family
  • 組織特異性：
    Keratinocytes of epidermis and other stratified squamous epithelia.
  • 數據庫鏈接：

    HGNC: 6187

    OMIM: 147360

    KEGG: hsa:3713

    STRING: 9606.ENSP00000357753

    UniGene: Hs.516439



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