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  4. Icam2 Recombinant Monoclonal Antibody

Icam2 Recombinant Monoclonal Antibody

  • 中文名稱：
    Icam2 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA759127A0MO
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HEK293 whole cell lysate(30μg), HEK293T whole cell lysate(30μg), Mouse lung tissue lysate(30μg)
      All lanes: ICAM 2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 31 kDa
      Observed band size: 60 kDa
      Exposure time：3min
    • IHC image of CSB-RA759127A0MO diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA759127A0MO diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Raji cells stained with CSB-RA759127A0MO (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Intercellular adhesion molecule 2 (ICAM-2) (Lymphocyte function-associated AG-1 counter-receptor) (CD antigen CD102), Icam2, Icam-2
  • 反應種屬：
    Mouse
  • 免疫原：
    A synthesized peptide derived from Mouse Icam2 protein
  • 免疫原種屬：
    Mus musculus (Mouse)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    20C6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • 基因功能參考文獻：
    1. Data show that intercellular adhesion molecule 1 (ICAM-1) and ICAM-2 on B cells are essential for long-lasting cognate T follicular helper (Tfh)-B cell interactions and efficient selection of low-affinity B cell clones for proliferative clonal expansion. PMID: 28939548
    2. Results indicate that the interaction of ICAM-2 with alpha-actinin is critical to conferring an ICAM-2-mediated non-metastatic phenotype in neuroblastoma cells. PMID: 24704826
    3. ICAM-2 regulates endothelial barrier function and permeability through a pathway involving N-Cadherin, ERMs and Rac-1 PMID: 24593809
    4. These findings highlight novel roles for ICAM-2 in mediating luminal neutrophil crawling and the effect on subsequent levels of extravasation. PMID: 24317296
    5. show that the sequential expression of CD40 and Icam2 delineate a transition in the acquisition of the blood potential from hemangioblast to hemogenic endothelium leading to the formation of primitive and definitive hematopoietic progenitors. PMID: 20506544
    6. Data report the crystal structure of the radixin FERM (4.1 and ERM) domain complexed with the intercellular adhesion molecule-2 (ICAM-2) cytoplasmic peptide. PMID: 12554651
    7. ICAM-2 and ICAM-1 have redundant functions in lymphocyte recirculation through lymph nodes, but ICAM-2 is not required for the migration of T effector cells into inflamed skin. PMID: 12928410
    8. The 60 kDa protein, the murine homologue of ICAM-2 (CD102), recognized by the BF/32 antibody was widely expressed on lymphocytes PMID: 18680764

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  • 亞細胞定位：
    Membrane; Single-pass type I membrane protein. Cell projection, microvillus.
  • 蛋白家族：
    Immunoglobulin superfamily, ICAM family
  • 組織特異性：
    Expressed in endothelial cells and leukocytes. High levels found in lung.
  • 數據庫鏈接：

    KEGG: mmu:15896

    STRING: 10090.ENSMUSP00000001055

    UniGene: Mm.394



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