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  4. LYPLA1 Recombinant Monoclonal Antibody

LYPLA1 Recombinant Monoclonal Antibody

  • 中文名稱：
    LYPLA1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA133174A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in:A-431 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), Mouse Brain whole cell lysate(30μg)
      All lanes: Lipoma preferred partner antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 25 kDa
      Observed band size: 25 kDa
      Exposure time：240s
    • Immunofluorescence staining of RAJI cell with CSB-RA133174A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Raji cells stained with CSB-RA133174A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    LYPLA1
  • 別名：
    Acyl-protein thioesterase 1 (APT-1) (hAPT1) (EC 3.1.2.-) (Lysophospholipase 1) (Lysophospholipase I) (LPL-I) (LysoPLA I), LYPLA1, APT1 LPL1
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from Human LYPLA1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    6B5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Acts as a acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Could also depalmitoylate ADRB2. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA.
  • 基因功能參考文獻：
    1. Data show taht active S-depalmitoylation in mitochondria, in part mediated by acyl-protein thioesterase 1 (APT1) PMID: 29362370
    2. Here, we describe the conserved functions of APT1 and APT2 across organisms and discuss the possibility that these enzymes are members of a larger family of depalmitoylation enzymes. PMID: 25849916
    3. High expression of APT1 is associated with chronic lymphocytic leukemia. PMID: 25670628
    4. identifcation APT1 as one of the thioesterases in the acylation cycle and demonstration that this protein is a cellular target of the inhibitor. PMID: 20418879
    5. Dynamic palmitoylation links cytosol-membrane shuttling of acyl-protein thioesterase-1 and acyl-protein thioesterase-2 with that of proto-oncogene H-ras product and growth-associated protein-43 PMID: 23396970
    6. Serum activity of APT1 may play an important role in determination of the concentration of des-acyl ghrelin in circulation, especially under septic inflammation. PMID: 20685872
    7. Endogenous and overexpressed hAPT1 were mainly localized in the cytosol, while some signals were detected in the plasma membrane, the nuclear membrane and endoplasmic reticulum in HEK293 cells. PMID: 19439193
    8. Results suggest that APT1-regulated depalmitoylation of Galpha(13) might be an important downstream event of miR-138 function. PMID: 19465924

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  • 亞細胞定位：
    Cytoplasm. Cell membrane. Nucleus membrane. Endoplasmic reticulum.
  • 蛋白家族：
    AB hydrolase superfamily, AB hydrolase 2 family
  • 組織特異性：
    Platelets.
  • 數據庫鏈接：

    HGNC: 6737

    OMIM: 605599

    KEGG: hsa:10434

    STRING: 9606.ENSP00000320043

    UniGene: Hs.744046



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