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  4. NCS1 Recombinant Monoclonal Antibody

NCS1 Recombinant Monoclonal Antibody

  • 中文名稱：
    NCS1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA944972A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Mouse brain tissue lysate(30μg), Rat brain tissue lysate(30μg)
      All lanes: NCS1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 22 kDa
      Observed band size: 22 kDa
      Exposure time：3min
    • IHC image of CSB-RA944972A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of hela cell with CSB-RA944972A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    NCS1
  • 別名：
    Neuronal calcium sensor 1 (NCS-1) (Frequenin homolog) (Frequenin-like protein) (Frequenin-like ubiquitous protein), NCS1, FLUP FREQ
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide derived from Human NCS1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    9H5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
  • 基因功能參考文獻：
    1. NCS-1, a calcium-binding protein, is associated with clinicopathologic features of aggressiveness in breast cancer cells and worse outcome in two breast cancer patient cohorts PMID: 28275088
    2. Results show that although NCS-1 Var2mRNA can be detected, protein expression was not detectable. When overexpressed in cell lines NCS-1 Var2 appears to be folded properly, yet it has a lower calcium affinity. Because most NCS-1 binding to its protein partners requires calcium binding, it is unlikely that NCS-1 Var2 would play a major regulatory role in cells. PMID: 27575489
    3. structural and dynamical response of NCS-1 protein to elevated temperature may be one of its intrinsic functional properties PMID: 27007011
    4. Lithium normalizes gamma band oscillations mediated by P/Q-type calcium channels modulated by NCS-1. PMID: 27033453
    5. Study determined the salient features of the folding free-energy landscapes of the Mg2+-bound and apo states of NCS-1 PMID: 26153708
    6. results reveal the important role of salt bridges on the structural properties of NCS-1 protein and that R102Q mutation disables the dynamic relocation of C-terminus, which may block the binding of NCS-1 protein to its receptors PMID: 25343687
    7. single-molecule optical tweezers were used to monitor misfolding reactions of the human neuronal calcium sensor-1. PMID: 25157171
    8. A strong liason was characterized between myristoylation and cryptic EF-1 in NCS-1. PMID: 25565019
    9. The functional link in substantia nigra dopamine neurons between Cav1.3- L-type-Ca2+ channels and D2-autoreceptor activity is an adaptive signalling network controlled by NCS-1. PMID: 24934288
    10. NCS1 has evolved a remarkable complex interdomain cooperativity and a fundamentally different folding mechanism compared to structurally related proteins. PMID: 24012477
    11. data suggest that genetic variants in the NCS-1 gene contribute to susceptibility of Cocaine Abuse in individuals of African descent. PMID: 22999924
    12. [review] Receptor interaction properties in schizophrenia are dependent upon NCS-1, a protein found to regulate the phosphorylation, trafficking, and signaling profile of the D2 dopamine receptor (D2R) in neurons. PMID: 21777187
    13. the C-terminal tail is important for regulating the conformational stability of NCS1 in Ca(2+)-activated state PMID: 22227393
    14. analysis of the interaction between the D2 dopamine receptor and neuronal calcium sensor-1 PMID: 21875085
    15. it is possible to protect cells from paclitaxel-induced degradation of NCS-1 by inhibiting calpain activity PMID: 21808066
    16. NCS-1 is a novel regulator of cardiac calcium signaling, specifically in immature and hypertrophic hearts. PMID: 21737792
    17. Impairment of the normal cycling of NCS-1 by the R102Q mutation could have subtle effects on neuronal signalling and physiology in the developing and adult brain. PMID: 20479890
    18. up-regulated in the prefrontal cortex of schizophrenic and bipolar patients PMID: 12496348
    19. Analysis with chimeric proteins between KChIP2 and NCS-1 reveals that the three regions of KChIP2 are necessary and sufficient for its effective binding to Kv4.3 protein PMID: 12928444
    20. may serve as regulator of certain isoforms of phosphatidylinositol 4-kinase PMID: 14512421
    21. key role for residues within the motif EELTRK in NCS-1 in keeping the myristoyl group exposed and allowing the protein to be constitutively membrane-associated. PMID: 14726528
    22. NCS-1 is shown to be mainly associated with azurophilic granules in human neutrophils and promyelocitic cells and, therefore could play an instrumental role in the calcium-dependent secretion of azurophilic granules PMID: 14760944
    23. These results demonstrate a novel role for NCS-1 and PI4Kbeta in regulating ERK1/2 signaling and inflammatory reactions in mast cells. PMID: 16837555
    24. NCS-1 expression was diminished in CD4+T lymphocytes, CD19+ B lymphocytes and CD14+ monocytes of bipolar disorder patients and also decreased in CD4+ T lymphocytes and CD56+ natural killer cells of schizophrenia patients PMID: 19091302
    25. occurrence of an unusual TG 3' splice site in intron 2 PMID: 17672918

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  • 亞細胞定位：
    Golgi apparatus. Cell junction, synapse, postsynaptic density. Cytoplasm, perinuclear region. Cytoplasm. Cell membrane; Peripheral membrane protein. Membrane; Lipid-anchor.
  • 蛋白家族：
    Recoverin family
  • 數據庫鏈接：

    HGNC: 3953

    OMIM: 603315

    KEGG: hsa:23413

    STRING: 9606.ENSP00000361475

    UniGene: Hs.642946



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