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  4. OSBPL1A Recombinant Monoclonal Antibody

OSBPL1A Recombinant Monoclonal Antibody

  • 中文名稱：
    OSBPL1A Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA870341A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: A431 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Rat brain tissue lysate(30μg)
      All lanes: ORP1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 50 kDa
      Observed band size: 50 kDa
      Exposure time：1min
    • IHC image of CSB-RA870341A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing A431 cells stained with CSB-RA870341A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    OSBPL1A
  • 別名：
    Oxysterol-binding protein-related protein 1 (ORP-1) (OSBP-related protein 1), OSBPL1A, ORP1 OSBP8 OSBPL1 OSBPL1B
  • 反應(yīng)種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide derived from Human OSBPL1A protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    7H6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Binds 25-hydroxycholesterol and cholesterol.
  • 基因功能參考文獻(xiàn)：
    1. ORP1L, via its liganding by lipids and the formation of contacts between autophagic vacuoles and the endoplasmic reticulum, governs the last steps in autophagy that lead to the lysosomal degradation of cytosolic material. PMID: 27283760
    2. This study validated that rs3746444 polymorphism influenced the expression of miR499a, its target gene, osbpl1a, and thereby associated with the HDL level, which makes it a potential factor involved in the mechanism of atherosclerosis. PMID: 29039586
    3. ORP1L-dependent membrane contacts between late endosomes/lysosomes and the endoplasmic reticulum coordinate cholesterol transfer with the retrograde movement of endo-lysosomal vesicles. PMID: 28564600
    4. a familial loss-of-function mutation in OSBPL1A affects the first step of the reverse cholesterol transport process and associates with a low HDL-C phenotype PMID: 27105157
    5. These data suggest that ORP1L is specifically recruited by the bacteria to the Coxiella parasitophorous vacuole, where it influences parasitophorous vacuole membrane dynamics and interactions with the endoplasmic reticulum. PMID: 27345457
    6. ORP1L-VAP complexes also support transport of LDL-derived cholesterol from endosomes to the endoplasmic reticulum when ORP1L was bound to human adenovirus RIDalpha. RIDalpha-induced lipid trafficking also attenuated proinflammatory signaling by Toll-like receptor 4, which has a central role in adenovirus pathogenesis and is known to be tightly regulated by cholesterol-rich "lipid rafts." PMID: 28077646
    7. Data suggest RIDalpha as a model system for understanding physiological egress routes that use ORP1L to activate endosome-to-endoplasmic reticulum (ER) feedback responses involved in lipid droplets (LDs)formation. PMID: 24025716
    8. ORP1S is a cytoplasmic sterol sensor, which transports sterols to the nucleus and promotes LXR regulated trans-activation of apoE. PMID: 22728266
    9. The study shows that OSBPL1A binds several oxysterols and cholesterol, and characterize a mutant, OSBPL1A Delta560-563, defective in oxysterol binding. PMID: 20690035
    10. Results suggest that the two forms of oxysterol binding protein-related protein-1 (ORP1) are functionally distinct and that ORP1L is involved in control of cellular lipid metabolism PMID: 12631712
    11. binds to Rab7, modifies its functional cycle, and can interfere with lysosome organization and endocytic membrane trafficking. PMID: 16176980
    12. OSBPL1A was preferentially expressed from the maternal allele PMID: 18369178

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  • 亞細(xì)胞定位：
    Late endosome. Note=Colocalizes with RAB7A, RAB9A and LAMP1 in late endosomes.
  • 蛋白家族：
    OSBP family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 16398

    OMIM: 606730

    KEGG: hsa:114876

    STRING: 9606.ENSP00000320291

    UniGene: Hs.370725



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