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  4. PGM1 Recombinant Monoclonal Antibody

PGM1 Recombinant Monoclonal Antibody

  • 中文名稱：
    PGM1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA965011A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in:PC-3 whole cell lysate(30μg), A431 whole cell lysate(30μg), Hela whole cell lysate(30μg), HepG2 whole cell lysate(30μg), BxPC-3 whole cell lysate(30μg), MCF7 whole cell lysate(30μg), 786-O whole cell lysate(30μg), Mouse heart tissue lysate(30μg), Rat heart tissue lysate(30μg)
      All lanes: PGM 1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 61 kDa
      Observed band size: 61 kDa
      Exposure time：15s
    • IHC image of CSB-RA965011A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of RAJI cell with CSB-RA965011A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA965011A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    PGM1
  • 別名：
    Phosphoglucomutase-1 (PGM 1) (EC 5.4.2.2) (Glucose phosphomutase 1), PGM1
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide derived from Human PGM1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    5H6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    This enzyme participates in both the breakdown and synthesis of glucose.
  • 基因功能參考文獻：
    1. PGM1 deficiency has been recognized as a cause of the congenital disorders of glycosylation. PMID: 29858906
    2. The structural perturbation resulting from mutation of this single amino acid reveals the molecular mechanism underlying PGM1 deficiency in these missense variants. PMID: 28117557
    3. Homozygous mutation in the PGM1 gene is associated with PGM1 deficiency. PMID: 26768186
    4. analysis of the structural basis of PGM1 enzyme dysfunction in PGM1 deficiency PMID: 26972339
    5. both PGM1 protein misfolding and catalytic impairment may play a role in PGM1 deficiency PMID: 25288802
    6. PGM1 is required for sustained cell growth during nutritional changes PMID: 24952355
    7. Phosphoglucomutase 1 deficiency, previously identified as a glycogenosis, is also a congenital disorder of glycosylation. PMID: 24499211
    8. The analysis involved the data on nine polymorphic codominant loci: HP, GC, TF, PI, PGM1, GLO1, C3, ACP1, and ESD. The loci were selected by significance of differences in genotype frequencies between tuberculosis patients and healthy controls PMID: 12942785
    9. Results report a novel human protein, calphoglin, which activates inorganic pyrophosphatase (IPP) and enhances phosphoglucomutase activity through the activated IPP. PMID: 15522220
    10. During extrauterine life, females carrying the PGM1*2 allele are relatively protected from extreme body mass increase, and during intrauterine life, PGM1*2/*2 homozygotes show a tendency to low body mass increase. PMID: 18091362
    11. There is a correlation between glomerular expression of MCP-1 and PGM1 and worsening renal prognosis in paediatric lupus nephritis. PMID: 18495743
    12. ZASP/Cypher anchors PGM1 to Z-disc under conditions of stress. The impaired binding of PGM1 to ZASP/Cypher might be involved in the pathogenesis of dilated cardiomyopathy. PMID: 19377068

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  • 相關疾病：
    Congenital disorder of glycosylation 1T (CDG1T)
  • 亞細胞定位：
    [Isoform 1]: Cytoplasm.
  • 蛋白家族：
    Phosphohexose mutase family
  • 數據庫鏈接：

    HGNC: 8905

    OMIM: 171900

    KEGG: hsa:5236

    UniGene: Hs.1869



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