哎呀哎呀在线观看视频|狼狼鲁色在线视频播放|天天躁日日躁狠狠躁欧美老妇|波斯女人1977满天星|九阳真经未满十八岁能学吗|被老外做的都肿了|7tv影院

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. TSFM Recombinant Monoclonal Antibody

TSFM Recombinant Monoclonal Antibody

  • 中文名稱：
    TSFM Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA153166A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(30μg), HEK293 whole cell lysate(30μg), COLO205 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), A549 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), Mouse kidney tissue lysate(30μg)
      All lanes: TSFM antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 35 kDa
      Observed band size: 32 kDa
      Exposure time：30s
    • IHC image of CSB-RA153166A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA153166A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of hela cell with CSB-RA153166A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing MCF-7? cells stained with CSB-RA153166A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    TSFM
  • 別名：
    Elongation factor Ts, mitochondrial (EF-Ts) (EF-TsMt), TSFM
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from Human TSFM protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    3D5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
  • 基因功能參考文獻：
    1. Different TSFM mutations can produce the same or very different clinical phenotypes, going from abortions to moderately severe presentations. On the other hand, the same TSFM mutation can also produce same or different phenotypes within the same range of presentations, therefore suggesting the involvement of unknown factors. PMID: 27677415
    2. show that in addition to early-onset cardiomyopathy, TSFM mutations should be considered in childhood and juvenile encephalopathies with optic and/or peripheral neuropathy, ataxia, or Leigh disease PMID: 25037205
    3. identified a homozygous mutation changing a highly conserved arginine into a tryptophan (R312W) in a kindred with intrauterine growth retardation, neonatal lactic acidosis, liver dysfunction and multiple respiratory chain deficiency in muscle PMID: 21741925
    4. Molecular modeling showed that the Arg333Trp substitution disrupts local subdomain structure and the dimerization interface. PMID: 17033963
  • 相關疾病：
    Combined oxidative phosphorylation deficiency 3 (COXPD3)
  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    EF-Ts family
  • 組織特異性：
    Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney.
  • 數據庫鏈接：

    HGNC: 12367

    OMIM: 604723

    KEGG: hsa:10102

    UniGene: Hs.632704



×
生命科學計算平臺