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  4. UFM1 Recombinant Monoclonal Antibody

UFM1 Recombinant Monoclonal Antibody

  • 中文名稱：
    UFM1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA217276A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HEK293 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), U251 whole cell lysate(30μg), CACO-2 whole cell lysate(30μg), PC-3 whole cell lysate(30μg)
      All lanes: UFM1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 9 kDa
      Observed band size: 9 kDa
      Exposure time：3min
    • Immunofluorescence staining of raji cell with CSB-RA217276A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing 786-O cells stained with CSB-RA217276A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    UFM1
  • 別名：
    Ubiquitin-fold modifier 1, UFM1, C13orf20
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human UFM1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    20G2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to lysine residues of substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress. Ufmylation of TRIP4 regulates nuclear receptors-mediated transcription.
  • 基因功能參考文獻(xiàn)：
    1. UFM1 His 70 resembles UBA5 His336 and enters a negatively charged pocked on the other UFM1 molecule. PMID: 28360427
    2. Ufmylation may participate in regulation of the vascular smooth muscle cells phenotypic switch and endothelial cell injury, which may help in the understanding of vascular remodeling. PMID: 29461087
    3. These findings explicitly elucidate the role of UBA5 dimerization in UFM1 activation. PMID: 27653677
    4. This study, focused on identifying the mutated gene in patients with hypomyelination with atrophy of the basal ganglia and cerebellum without TUBB4A mutations, revealed a homozygous 3-bp deletion in the UFM1 promoter area, which perfectly segregates with the disease. PMID: 28931644
    5. Meta analysis identified a new gene, UFM1, that is associated with low von Willebrand factor levels. PMID: 26486471
    6. in the absence of UFSP2, a deconjugating enzyme for UFM1, ectopic expression of both UFL1 and UFBP1, which serve as the E3-ligase complex for the UFM1-system, dramatically increases UFM1-conjugate formation at the endoplasmic reticulum. PMID: 27926783
    7. data imply that the combination of a hypomorphic p.Ala371Thr variant in trans with a loss-of-function allele in UBA5 underlies a severe infantile-onset encephalopathy PMID: 27545674
    8. Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy PMID: 27545681
    9. results of an in vitro truncation assay suggest that Uba5 residues 57-363 comprise the minimal fragment required for the high-efficiency activation of Ufm1 PMID: 24915089
    10. ASC1 is a target for ufmylation and that UFBP1 is an essential component for ASC1 ufmylation. PMID: 25219498
    11. binding of ATP to Uba5 approximately Ufm1 thioester was required for efficient transfer of Ufm1 from Uba5 to Ufc1 via transthiolation. PMID: 24966333
    12. Thus, UfSP2 proteins have protease- and Ufm1-independent functions in GPCR biogenesis. PMID: 24603482
    13. the Ufm1 system plays a critical role in vesicle trafficking, and its inhibition may lead to protein overload in the ER and UPR activation. PMID: 23152784
    14. A novel type of E3 ligase for the Ufm1 conjugation system. PMID: 20018847
    15. The solution structure and dynamics of human Ufm1 (hsUfm1) by nuclear magnetic resonance spectroscopy was analysed. PMID: 16527251

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    UFM1 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 20597

    OMIM: 610553

    KEGG: hsa:51569

    STRING: 9606.ENSP00000239878

    UniGene: Hs.643655



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