(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, Mouse Heart tissue, Mouse Brain tissue
All lanes: ATP5F1A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa

IHC image of CSB-RA159926A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA159926A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: K562 whole cell lysate, Mouse heart tissue
All lanes: ATP5F1A antibody at 2.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa

Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA002344HA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002344HA01HU at dilution of 1:100

Immunoprecipitating ATP5A1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA002344HA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA002344HA01HU (4ug) + Hela whole cell lysate (500ug)
Lane 3: Hela whole cell lysate (20ug)

Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Mouse brain tissue, Rat brain tissue
All lanes: ATP5A1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa