(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of BUB3 expression in HepG2 cells using BUB3 antibody. Green, isotype control; red, BUB3.

Immunocytochemical staining of HepG2 cells with BUB3 antibody. Nuclei were stained blue with DAPI; BUB3 was stained magenta with Alexa Fluor? 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using BUB3 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BUB3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue
All lanes: BUB3 antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38, 37 kDa
Observed band size: 38, 37 kDa

Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA002884LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA002884LA01HU at dilution of 1:100