(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of calpain 1 expression in C2C12 cells using calpain 1 antibody. Green, isotype control; red, calpain 1.

Immunocytochemical staining of C2C12 cells with Calpain 1?antibody. Nuclei were stained blue with DAPI; Calpain 1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using calpain 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpain 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blot
All lanes: Calpain-1 catalytic subunit antibody at 2μg/ml
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa

Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA004490ESR2HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA004490ESR2HU at dilution of 1:100