(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP004547HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CASP2.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP004547HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CASP2.

Western Blot analysis of K562 cells using Caspase-2 Polyclonal Antibody

Western Blot analysis of K562 cells using Caspase-2 Polyclonal Antibody

Flow cytometric analysis of Caspase 2 expression in HepG2 cells using Caspase 2 antibody. Green, isotype control; red, Caspase 2.

Immunocytochemical staining of HepG2 cells with Caspase 2 antibody. Nuclei were stained blue with DAPI; Caspase 2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using caspase 2 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.