(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of Caspase 8 expression in HepG2 cells using Caspase 8 antibody. Green, isotype control; red, Caspase 8.

Immunocytochemical staining of HepG2 cells with Caspase 8 antibody. Nuclei were stained blue with DAPI; Caspase 8 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using caspase 8 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blot
All lanes: CASP8 antibody at 2µg/ml
Lane 1: ACCM whole cell lysate
Lane 2: LO2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 56, 54, 46, 58, 28, 26, 33, 31, 62 kDa
Observed band size: 56 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA18109A0Rb at dilution of 1:20

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA18109A0Rb at dilution of 1:20

Immunofluorescent analysis of HepG2 cells using CSB-PA18109A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)