(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: PC-3 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), HeLa whole cell lysate(30μg), HEK293 whole cell lysate(30μg), A549 whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), HepG2 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
All lanes: CDK5 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 33 kDa
Exposure time: 2min

Immunofluorescence staining of Hela cell with CSB-RA219236A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing A431 cells stained with CSB-RA219236A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot
Positive WB detected in: Mouse heart tissue, Mouse brain tissue
All lanes: CDK5 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 30 kDa
Observed band size: 34 kDa

Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA005067LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA005067LA01HU at dilution of 1:100

Immunofluorescent analysis of PC-3 cells using CSB-PA005067LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)