(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of COXIV expression in HepG2 cells using COXIV antibody. Green, isotype control; red, COXIV.

Immunocytochemical staining of HepG2 cells with COXIV antibody. Nuclei were stained blue with DAPI; COXIV was stained magenta with Alexa Fluor? 647.Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using COXIV antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using COXIV antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with COXIV antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blot
All lanes: COX4I1 antibody at 10µg/ml
Lane 1: Rat muscle tissue
Lane 2: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa

Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA00509A0Rb at dilution of 1:100