Western Blot
Positive WB detected in: JK whole cell lysate(30μg), SY5Y whole cell lysate(30μg), U251 whole cell lysate(30μg), K562 whole cell lysate(30μg), U87 whole cell lysate(30μg), HepG2 whole cell lysate(30μg),Mouse Liver tissue lysate(30μg)，Rat Liver tissue lysate(30μg)
All lanes: CYP4V2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 61,59 kDa
Observed band size: 61,59 kDa
Exposure time: 120s

IHC image of CSB-PA765094ESR2HU diluted at 1:50 and staining in paraffin-embedded human Liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA765094ESR2HU diluted at 1:50 and staining in paraffin-embedded human Liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody