(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of Endothelin receptor type B expression in HepG2 cells using Endothelin receptor type B antibody. Green, isotype control; red, Endothelin receptor type B.

Immunocytochemical staining of HepG2 cells with Endothelin receptor type B1 antibody. Nuclei were stained blue with DAPI; Endothelin receptor type B was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using Endothelin receptor type B antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Endothelin receptor type B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western Blot
Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue
All lanes: EDNRB antibody at 4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 49, 60 kDa
Observed band size: 50 kDa

Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA007404LA01HU at dilution of 1:100

Immunofluorescent analysis of PC-3 cells using CSB-PA007404LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)