(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of Eukaryotic translation elongation factor 2 expression in HepG2 cells using Eukaryotic translation elongation factor 2 antibody. Green, isotype control; red, Eukaryotic translation elongation factor 2.

Immunocytochemical staining of HepG2 cells with Eukaryotic translation elongation factor 2 antibody. Nuclei were stained blue with DAPI; Eukaryotic translation elongation factor 2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using eukaryotic translation elongation factor 2 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using eukaryotic translation elongation factor 2 antibody. Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with eukaryotic translation elongation factor 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA007434LA01HU at dilution of 1:50

Immunofluorescent analysis of HepG2 cells using CSB-PA007434LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)