(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of EIF2AK2 expression in HepG2 cells using EIF2AK2 antibody. Green, isotype control; red,EIF2AK2.

Immunocytochemical staining of HepG2 cells with EIF2AK2 antibody. Nuclei were stained blue with DAPI; EIF2AK2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using EIF2AK2 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF2AK2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. EIF2AK2, eukaryotic translation initiation factor 2 alpha kinase 2.

Western Blot analysis of HeLa cells using PKR Polyclonal Antibody

Western Blot analysis of NIH-3T3 cells using PKR Polyclonal Antibody